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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-8-20
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pubmed:databankReference |
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pubmed:abstractText |
The genome organization and expression strategy of the newly identified severe acute respiratory syndrome coronavirus (SARS-CoV) were predicted using recently published genome sequences. Fourteen putative open reading frames were identified, 12 of which were predicted to be expressed from a nested set of eight subgenomic mRNAs. The synthesis of these mRNAs in SARS-CoV-infected cells was confirmed experimentally. The 4382- and 7073 amino acid residue SARS-CoV replicase polyproteins are predicted to be cleaved into 16 subunits by two viral proteinases (bringing the total number of SARS-CoV proteins to 28). A phylogenetic analysis of the replicase gene, using a distantly related torovirus as an outgroup, demonstrated that, despite a number of unique features, SARS-CoV is most closely related to group 2 coronaviruses. Distant homologs of cellular RNA processing enzymes were identified in group 2 coronaviruses, with four of them being conserved in SARS-CoV. These newly recognized viral enzymes place the mechanism of coronavirus RNA synthesis in a completely new perspective. Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
331
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
991-1004
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12927536-Amino Acid Sequence,
pubmed-meshheading:12927536-Animals,
pubmed-meshheading:12927536-Cercopithecus aethiops,
pubmed-meshheading:12927536-Conserved Sequence,
pubmed-meshheading:12927536-Coronavirus,
pubmed-meshheading:12927536-Evolution, Molecular,
pubmed-meshheading:12927536-Genome, Viral,
pubmed-meshheading:12927536-Humans,
pubmed-meshheading:12927536-Molecular Sequence Data,
pubmed-meshheading:12927536-Open Reading Frames,
pubmed-meshheading:12927536-Phylogeny,
pubmed-meshheading:12927536-Protein Structure, Tertiary,
pubmed-meshheading:12927536-Protein Subunits,
pubmed-meshheading:12927536-Proteome,
pubmed-meshheading:12927536-RNA, Messenger,
pubmed-meshheading:12927536-RNA, Viral,
pubmed-meshheading:12927536-RNA Processing, Post-Transcriptional,
pubmed-meshheading:12927536-RNA Replicase,
pubmed-meshheading:12927536-SARS Virus,
pubmed-meshheading:12927536-Sequence Homology, Amino Acid,
pubmed-meshheading:12927536-Vero Cells,
pubmed-meshheading:12927536-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
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pubmed:affiliation |
Department of Medical Microbiology, Leiden University Medical Center, Room L4-34, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study
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