Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25-26
pubmed:dateCreated
2003-8-18
pubmed:abstractText
Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositions and adjuvanticity. The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-gamma, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3961-71
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12922132-Adjuvants, Immunologic, pubmed-meshheading:12922132-Alum Compounds, pubmed-meshheading:12922132-Animals, pubmed-meshheading:12922132-Antigens, pubmed-meshheading:12922132-Carbohydrate Sequence, pubmed-meshheading:12922132-Cell Line, pubmed-meshheading:12922132-Chromatography, Liquid, pubmed-meshheading:12922132-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:12922132-Female, pubmed-meshheading:12922132-Immunity, Cellular, pubmed-meshheading:12922132-Immunoglobulin G, pubmed-meshheading:12922132-Indicators and Reagents, pubmed-meshheading:12922132-Interferon-gamma, pubmed-meshheading:12922132-Interleukin-2, pubmed-meshheading:12922132-Mass Spectrometry, pubmed-meshheading:12922132-Mice, pubmed-meshheading:12922132-Mice, Inbred BALB C, pubmed-meshheading:12922132-Mice, Inbred C57BL, pubmed-meshheading:12922132-Molecular Sequence Data, pubmed-meshheading:12922132-Ovalbumin, pubmed-meshheading:12922132-Quillaja, pubmed-meshheading:12922132-Saponins, pubmed-meshheading:12922132-Th1 Cells
pubmed:year
2003
pubmed:articleTitle
Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100.
pubmed:affiliation
Galenica Pharmaceuticals Inc., 2800 Milan Court, Suite 118, Birmingham, AL 35211, USA. marciani.gpi@att.net
pubmed:publicationType
Journal Article