Source:http://linkedlifedata.com/resource/pubmed/id/12922103
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
25-26
|
| pubmed:dateCreated |
2003-8-18
|
| pubmed:abstractText |
VP1, a capsid protein of foot-and-mouth disease virus (FMDV), contains neutralizing epitopes of the virus. Due to its poor water solubility, recombinant Escherichia coli derived VP1 (rVP1) has previously been used mainly in a denatured form and is not well characterized. Here, using SDS to assist protein refolding and then removing SDS with a detergent removing column, we have successfully purified rVP1 in two aqueous-soluble forms, i.e. monomer and dimer. Studies showed that dimerization occurs by an inter-molecular disulfide bond between two cysteine residues at position 187 of each monomer. Heat treatment revealed that rVP1 dimer exhibited a more thermal-stable conformation than the monomeric form. Both monomeric and dimeric rVP1 reacted with anti-FMDV antibodies. Immunization studies demonstrated that vaccination of swine with either forms of rVP1 was effective in generating immune responses and protecting them from viral challenge.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0264-410X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
8
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3721-9
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12922103-Amino Acid Sequence,
pubmed-meshheading:12922103-Animals,
pubmed-meshheading:12922103-Antibodies, Viral,
pubmed-meshheading:12922103-Biophysical Phenomena,
pubmed-meshheading:12922103-Biophysics,
pubmed-meshheading:12922103-Cell Division,
pubmed-meshheading:12922103-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12922103-Escherichia coli,
pubmed-meshheading:12922103-Foot-and-Mouth Disease,
pubmed-meshheading:12922103-Foot-and-Mouth Disease Virus,
pubmed-meshheading:12922103-Hot Temperature,
pubmed-meshheading:12922103-Immunization,
pubmed-meshheading:12922103-Molecular Sequence Data,
pubmed-meshheading:12922103-Neutralization Tests,
pubmed-meshheading:12922103-Protein Folding,
pubmed-meshheading:12922103-Swine,
pubmed-meshheading:12922103-Swine Diseases,
pubmed-meshheading:12922103-T-Lymphocytes,
pubmed-meshheading:12922103-Vaccines, DNA,
pubmed-meshheading:12922103-Vaccines, Synthetic,
pubmed-meshheading:12922103-Viral Vaccines
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Induction of immunity in swine by purified recombinant VP1 of foot-and-mouth disease virus.
|
| pubmed:affiliation |
Institute of Bioagricultural Sciences, Academia Sinica, No. 128 Academia Road, Section 2 Nankang, Taipei 11529, Taiwan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|