Linked Life Data

12919485

Source:http://linkedlifedata.com/resource/pubmed/id/12919485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-8-15
pubmed:abstractText
In order to examine the usefulness of detoxifying genes as molecular markers in different chemical environments, isolation of cytochrome P450 genes (CYPs) belonging to the CYP4 family was performed in different samples from two subalpine populations of Daphnia pulex. The use of degenerate primers allowed us to isolate seven cDNAs. Four of them were assigned to the CYP4C subfamily, and were closely related to previously isolated crustacean CYP4s while the others were assigned to new CYP4AN and CYP4AP subfamilies. Expression studies, using semiquantitative polymerase chain reaction (PCR) followed by Southern blot hybridization with specific probes revealed differences in CYP4C32 and CYP4AP1 expressions between the two populations, which differ in the polyphenol richness of the vegetation surrounding their aquatic habitat. Further exposure to toxic dietary polyphenols showed different CYP induction patterns. Taken together, these preliminary results suggest a possible involvement of CYP4s in the ecological differentiation of subalpine D. pulex populations related to the polyphenol richness of the environmental vegetation. CYP4s may thus be considered as possible molecular markers in aquatic environmental bioreporting.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0962-1083
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2473-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12919485-Amino Acid Sequence, pubmed-meshheading:12919485-Animals, pubmed-meshheading:12919485-Blotting, Southern, pubmed-meshheading:12919485-Cytochrome P-450 Enzyme System, pubmed-meshheading:12919485-DNA Primers, pubmed-meshheading:12919485-Daphnia, pubmed-meshheading:12919485-Environmental Monitoring, pubmed-meshheading:12919485-Flavonoids, pubmed-meshheading:12919485-Fresh Water, pubmed-meshheading:12919485-Gene Expression Profiling, pubmed-meshheading:12919485-Gene Expression Regulation, pubmed-meshheading:12919485-Molecular Sequence Data, pubmed-meshheading:12919485-Phenols, pubmed-meshheading:12919485-Phylogeny, pubmed-meshheading:12919485-Polyphenols, pubmed-meshheading:12919485-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12919485-Sequence Analysis, DNA
pubmed:year
2003
pubmed:articleTitle
Molecular approach to aquatic environmental bioreporting: differential response to environmental inducers of cytochrome P450 monooxygenase genes in the detritivorous subalpine planktonic Crustacea, Daphnia pulex.
pubmed:affiliation
Laboratoire Ecosystèmes et Changements Environnementaux, EA 3112, Centre de Biologie Alpine, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't