Linked Life Data

12918975

Source:http://linkedlifedata.com/resource/pubmed/id/12918975

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2003-8-15
pubmed:abstractText
The use of microfabricated cantilevers as bioaffinity sensors was investigated. Since many bioaffinity interactions involve proteins as receptors, we conducted studies of the magnitude, kinetics, and reversibility of surface stresses caused when common proteins interact with microcantilevers (MCs) with nanostructured (roughened) gold surfaces on one side. Exposure of nanostructured, unfunctionalized MCs to the proteins immunoglobulin G and bovine serum albumin (BSA) resulted in reversible large tensile stresses, whereas MCs with smooth gold surfaces on one side produced reversible responses that were considerably smaller and compressive. The response magnitude for nanostructured MCs exposed to BSA is shown to be concentration dependent, and linear calibration over the range of 1-200 mg/L is demonstrated. Stable, reusable protein bioaffinity phases based on unique enantioselective antibodies are created by covalently linking monoclonal antibodies to nanostructured MC surfaces. The direct (label-free) stereoselective detection of trace amounts of an important class of chiral analytes, the alpha-amino acids, was achieved based on immunomechanical responses involving nanoscale bending of the cantilever. The temporal response of the cantilever (delta deflection/delta time) is linearly proportional to the analyte concentration and allows the quantitative determination of enantiomeric purity up to an enantiomeric excess of 99.8%. To our knowledge, this is the first demonstration of chiral discrimination using highly scalable microelectromechanical systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2342-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Enantioselective sensors based on antibody-mediated nanomechanics.
pubmed:affiliation
Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.