Linked Life Data

12917473

Source:http://linkedlifedata.com/resource/pubmed/id/12917473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 9
pubmed:dateCreated
2003-8-14
pubmed:databankReference
pubmed:abstractText
The most abundantly transcribed HCMV gene (beta 2.7) encodes a 2.7 kb polyadenylated RNA. Although the laboratory-adapted HCMV strains AD169 and Towne possess two copies of the beta 2.7 gene within an expanded b sequence element, the low passage strain Toledo and all clinical isolates analysed contain only a single copy located within the U(L) region. A beta 2.7 deletion mutant constructed based on a strain Toledo background was shown to replicate with kinetics comparable to those of the parental virus; the beta2.7 gene is therefore not essential for virus replication in vitro. Sequencing the beta 2.7 gene from HCMV clinical isolates and the Toledo strain reveals that although the overall gene sequence is highly conserved (>99 %), the RL4 frame originally assigned in strain AD169 was disrupted in each of these viruses. Consequently, the beta 2.7 transcript does not encode any obvious translation product and thus may not function as an mRNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2511-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro.
pubmed:affiliation
Section of Infection and Immunity, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XY, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't