Source:http://linkedlifedata.com/resource/pubmed/id/12912949
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2003-8-12
|
| pubmed:abstractText |
We evaluated whether detection of human telomerase reverse transcriptase (hTERT) mRNA after immunomagnetic separation is useful to detect circulating cancer (CC) cells. Experimental Design: Two ml of peripheral blood were collected from 55 cases with hepatocellular carcinoma (HCC), 20 cases with chronic liver diseases devoid of cancer, and 20 healthy volunteers. Then 1500 and 500 micro l were subjected to immunomagnetic separations using Ber-EP4 and anti-CD45 antibodies, harvested and supernatant cells were collected as epithelial and nonleukocyte fractions, respectively. Samples of each fraction were subjected to reverse transcription-PCR detecting beta-actin, interleukin-2 receptor (IL-2r), alpha-fetoprotein, and hTERT mRNAs. The cases were judged to be positive, equivocal, or negative for CC cells when hTERT positivity with IL-2r negativity, hTERT positivity with IL-2r positivity, or hTERT negativity was seen in epithelial and/or nonleukocyte fractions, respectively.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1078-0432
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3004-11
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12912949-Aged,
pubmed-meshheading:12912949-Antigens, CD45,
pubmed-meshheading:12912949-Blood Cell Count,
pubmed-meshheading:12912949-Carcinoma, Hepatocellular,
pubmed-meshheading:12912949-Cell Separation,
pubmed-meshheading:12912949-DNA-Binding Proteins,
pubmed-meshheading:12912949-Female,
pubmed-meshheading:12912949-HeLa Cells,
pubmed-meshheading:12912949-Hepatocytes,
pubmed-meshheading:12912949-Humans,
pubmed-meshheading:12912949-Immunomagnetic Separation,
pubmed-meshheading:12912949-Liver Neoplasms,
pubmed-meshheading:12912949-Male,
pubmed-meshheading:12912949-Middle Aged,
pubmed-meshheading:12912949-Neoplasm Metastasis,
pubmed-meshheading:12912949-Neoplastic Cells, Circulating,
pubmed-meshheading:12912949-Prognosis,
pubmed-meshheading:12912949-RNA, Messenger,
pubmed-meshheading:12912949-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12912949-Sensitivity and Specificity,
pubmed-meshheading:12912949-Telomerase
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Sensitive and specific detection of circulating cancer cells in patients with hepatocellular carcinoma; detection of human telomerase reverse transcriptase messenger RNA after immunomagnetic separation.
|
| pubmed:affiliation |
Division of Gastroenterology and Hepatology, Department of Cellular Function, Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8122, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|