Linked Life Data

12911748

Source:http://linkedlifedata.com/resource/pubmed/id/12911748

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-8-12
pubmed:abstractText
The possible localization of cannabinoid (CB) receptors to glutamatergic and GABAergic synaptic terminals impinging upon GABAergic interneurons in the CA1 region of the rat hippocampus was examined using the electrophysiological measurement of neurotransmitter release in brain slices. Whereas activation of cannabinoid receptors via the application of the cannabinoid agonist WIN55,212-2 significantly and dose-dependently reduced evoked IPSCs recorded from interneurons possessing somata located in the stratum radiatum (S.R.) and stratum oriens (S.O.) lamellae, evoked glutamatergic EPSCs were unaffected in both neuronal populations. However, in agreement with previous reports, WIN55,212-2 significantly reduced EPSCs recorded from CA1 pyramidal neurons. Additional experiments confirmed that the effects of WIN55,212-2 on IPSCs were presynaptic and that they could be blocked by the CB1 receptor antagonist SR141716A. The involvement of endogenous cannabinoids in the presynaptic inhibition of GABA release was also examined in the interneurons and pyramidal cells using a depolarization-induced suppression of inhibition (DSI) paradigm. DSI was observed in CA1 pyramidal neurons under control conditions, and its incidence was greatly increased by the cholinergic agonist carbachol. However, DSI was not observed in the S.R. or S.O. interneuron populations, in either the presence or absence of carbachol. Whereas DSI was not present in these interneurons, the inhibitory inputs to these cells were modulated by the synthetic cannabinoid WIN55,212-2. These data support the hypothesis that cannabinoid receptors are located on inhibitory, but not excitatory, axon terminals impinging upon hippocampal interneurons, and that CA1 pyramidal neurons, and not interneurons, are capable of generating endogenous cannabinoids during prolonged states of depolarization.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
524-34
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12911748-Animals, pubmed-meshheading:12911748-Benzoxazines, pubmed-meshheading:12911748-Cannabinoids, pubmed-meshheading:12911748-Electrophysiology, pubmed-meshheading:12911748-Excitatory Postsynaptic Potentials, pubmed-meshheading:12911748-Glutamic Acid, pubmed-meshheading:12911748-Hippocampus, pubmed-meshheading:12911748-Interneurons, pubmed-meshheading:12911748-Male, pubmed-meshheading:12911748-Morpholines, pubmed-meshheading:12911748-Naphthalenes, pubmed-meshheading:12911748-Neural Inhibition, pubmed-meshheading:12911748-Neurons, pubmed-meshheading:12911748-Piperidines, pubmed-meshheading:12911748-Pyramidal Cells, pubmed-meshheading:12911748-Pyrazoles, pubmed-meshheading:12911748-Rats, pubmed-meshheading:12911748-Rats, Sprague-Dawley, pubmed-meshheading:12911748-Receptors, Cannabinoid, pubmed-meshheading:12911748-Receptors, Drug, pubmed-meshheading:12911748-Synapses, pubmed-meshheading:12911748-Tissue Distribution, pubmed-meshheading:12911748-gamma-Aminobutyric Acid
pubmed:year
2003
pubmed:articleTitle
Functional localization of cannabinoid receptors and endogenous cannabinoid production in distinct neuron populations of the hippocampus.
pubmed:affiliation
Cellular Neurobiology Research Branch, National Institute on Drug Abuse, National Institutes of Health, US Department of Health and Human Services, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.