Linked Life Data

1291064

Source:http://linkedlifedata.com/resource/pubmed/id/1291064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-4-1
pubmed:abstractText
The HT-29 cell line derived from a human colon adenocarcinoma has a glucose-dependent state of differentiation which is negatively correlated with the presence of D-glucose in the culture medium. The contribution of glucose to the biosynthesis of N-glycan chains, as a function of the differentiation state of HT-29 cells, was shown by: (a) [14C]glucose incorporation by undifferentiated HT-29 cells being lower after 2 h and higher after 19 h of metabolic labeling than that by differentiated cells; (b) a lack of glucose in the culture medium of undifferentiated HT-29 cells diminishing [14C]glucose incorporation into glycan chains, but not changing the glucose distribution between lipid- and protein-linked saccharides; (c) glucose behavior in undifferentiated HT-29 cells being not related to mannose-glycan metabolism, as the high-mannose compounds labeled with glucose and observed by HPLC showing a different distribution associated with the duration of glucose labeling; and (d) glucose being interconverted into other monosaccharide-glycan constituents in proportions different in differentiated and undifferentiated cell populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0008-6215
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
236
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-105
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Relationship between the content of [14C]glucose-derived monosaccharides in glycoprotein oligosaccharide chains and the state of enterocytic differentiation of HT-29 cells.
pubmed:affiliation
INSERM U 180, UFR Biomédicale des Saints-Pères, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't