Linked Life Data

12909585

Source:http://linkedlifedata.com/resource/pubmed/id/12909585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-10-10
pubmed:abstractText
Fibulin-5, previously known as DANCE and EVEC, is a secreted extracellular matrix protein that functions as a scaffold for elastin fiber assembly and as a ligand for integrins alphavbeta3, alphavbeta5, and alpha9beta1. Fibulin-5 is developmentally regulated in the lung, and lung air space enlargement develops in mice deficient in fibulin-5. Fibulin-5 is also induced in adult lung following lung injury by hyperoxia. To further examine the role of fibulin-5 during repair of lung injury, we assessed fibulin-5 expression during elastase-induced emphysema in C57/b mice. Mice were treated with either saline or elastase via the trachea, and the lung was examined 20 days after treatment. Fibulin-5 mRNA was induced almost fourfold, whereas elastin mRNA was minimally elevated. Immunohistochemistry studies showed that fibulin-5 was induced in cells within the alveolar wall following elastase treatment. Western analysis demonstrates that fibulin-5 was strongly expressed in isolated primary lung interstitial fibroblasts. Fibulin-5 protein was localized to the fibroblast cell layer in culture, and brief elastase treatment degraded the protein. Intact fibulin-5 did not accumulate in the culture media. Treatment of fibroblasts with the proinflammatory cytokine interleukin-1beta abolished fibulin-5 mRNA expression. Our results indicate that fibulin-5 is coordinately expressed and regulated with elastin in lung fibroblasts and may serve a key role during lung injury and repair.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1040-0605
pubmed:author
pubmed:issnType
Print
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L1147-52
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12909585-Amino Acid Sequence, pubmed-meshheading:12909585-Animals, pubmed-meshheading:12909585-Cells, Cultured, pubmed-meshheading:12909585-Elastin, pubmed-meshheading:12909585-Extracellular Matrix Proteins, pubmed-meshheading:12909585-Fibroblasts, pubmed-meshheading:12909585-Gene Expression Regulation, pubmed-meshheading:12909585-Hyperoxia, pubmed-meshheading:12909585-Integrins, pubmed-meshheading:12909585-Ligands, pubmed-meshheading:12909585-Lung, pubmed-meshheading:12909585-Lung Injury, pubmed-meshheading:12909585-Mice, pubmed-meshheading:12909585-Mice, Inbred C57BL, pubmed-meshheading:12909585-Mice, Knockout, pubmed-meshheading:12909585-Pancreatic Elastase, pubmed-meshheading:12909585-Peptide Fragments, pubmed-meshheading:12909585-RNA, Messenger, pubmed-meshheading:12909585-Rats, pubmed-meshheading:12909585-Recombinant Proteins, pubmed-meshheading:12909585-Transcription, Genetic
pubmed:year
2003
pubmed:articleTitle
Coordinate expression of fibulin-5/DANCE and elastin during lung injury repair.
pubmed:affiliation
The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.