| pubmed-article:12906102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C0445795 | lld:lifeskim |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C0598888 | lld:lifeskim |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C0182537 | lld:lifeskim |
| pubmed-article:12906102 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:12906102 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:12906102 | pubmed:dateCreated | 2003-8-8 | lld:pubmed |
| pubmed-article:12906102 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12906102 | pubmed:abstractText | We cloned a DNA fragment responsible for drug resistance from chromosome of Vibrio cholerae non-O1. Nucleotide sequence analysis of this fragment revealed the presence of a single open reading frame encoding a protein consisting of 445 amino acid residues. We designated the gene as vcrM. Hydropathy analysis of the deduced amino acid sequence of VcrM suggests the presence of 12 trans-membrane segments. A dendrogram showed that VcrM is a member of the DinF-subfamily within the MATE family of multidrug efflux pumps. Expression of the cloned vcrM gene in drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4', 6-diamidino-2-phenylindole, Hoechst 33342, rhodamine 6G, tetraphenylphosphonium chloride (TPPCl) and ethidium bromide. Efflux of acriflavine due to VcrM was dependent on Na+ or Li+. Moreover, Na+ efflux was observed with VcrM when TPPCl was added to Na+-loaded cells. Therefore, we conclude that VcrM is a Na+/drug antiporter-type multidrug efflux pump. | lld:pubmed |
| pubmed-article:12906102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12906102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12906102 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12906102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12906102 | pubmed:issn | 0385-5600 | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:TsuchiyaTomof... | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:ChenJingJ | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:MoritaYujiY | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:KurodaTeruoT | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:MizushimaTohr... | lld:pubmed |
| pubmed-article:12906102 | pubmed:author | pubmed-author:HudaMd... | lld:pubmed |
| pubmed-article:12906102 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12906102 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:12906102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12906102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12906102 | pubmed:pagination | 419-27 | lld:pubmed |
| pubmed-article:12906102 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12906102 | pubmed:meshHeading | pubmed-meshheading:12906102... | lld:pubmed |
| pubmed-article:12906102 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12906102 | pubmed:articleTitle | Gene cloning and characterization of VcrM, a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1. | lld:pubmed |
| pubmed-article:12906102 | pubmed:affiliation | Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, Okayama 700-8530, Japan. | lld:pubmed |
| pubmed-article:12906102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12906102 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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