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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-8-8
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pubmed:databankReference |
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pubmed:abstractText |
We cloned a DNA fragment responsible for drug resistance from chromosome of Vibrio cholerae non-O1. Nucleotide sequence analysis of this fragment revealed the presence of a single open reading frame encoding a protein consisting of 445 amino acid residues. We designated the gene as vcrM. Hydropathy analysis of the deduced amino acid sequence of VcrM suggests the presence of 12 trans-membrane segments. A dendrogram showed that VcrM is a member of the DinF-subfamily within the MATE family of multidrug efflux pumps. Expression of the cloned vcrM gene in drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4', 6-diamidino-2-phenylindole, Hoechst 33342, rhodamine 6G, tetraphenylphosphonium chloride (TPPCl) and ethidium bromide. Efflux of acriflavine due to VcrM was dependent on Na+ or Li+. Moreover, Na+ efflux was observed with VcrM when TPPCl was added to Na+-loaded cells. Therefore, we conclude that VcrM is a Na+/drug antiporter-type multidrug efflux pump.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0385-5600
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
419-27
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12906102-Acriflavine,
pubmed-meshheading:12906102-Amino Acid Sequence,
pubmed-meshheading:12906102-Anti-Bacterial Agents,
pubmed-meshheading:12906102-Antiporters,
pubmed-meshheading:12906102-Biological Transport,
pubmed-meshheading:12906102-Cations, Monovalent,
pubmed-meshheading:12906102-Cloning, Molecular,
pubmed-meshheading:12906102-Drug Resistance, Multiple, Bacterial,
pubmed-meshheading:12906102-Escherichia coli,
pubmed-meshheading:12906102-Genes, Bacterial,
pubmed-meshheading:12906102-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:12906102-Microbial Sensitivity Tests,
pubmed-meshheading:12906102-Molecular Sequence Data,
pubmed-meshheading:12906102-Multigene Family,
pubmed-meshheading:12906102-Phylogeny,
pubmed-meshheading:12906102-Recombinant Fusion Proteins,
pubmed-meshheading:12906102-Sequence Alignment,
pubmed-meshheading:12906102-Sequence Homology, Amino Acid,
pubmed-meshheading:12906102-Sodium,
pubmed-meshheading:12906102-Vibrio cholerae
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pubmed:year |
2003
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pubmed:articleTitle |
Gene cloning and characterization of VcrM, a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1.
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pubmed:affiliation |
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, Okayama 700-8530, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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