Linked Life Data

12904474

Source:http://linkedlifedata.com/resource/pubmed/id/12904474

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2003-8-7
pubmed:abstractText
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
6
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7143-54
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12904474-Animals, pubmed-meshheading:12904474-Brain, pubmed-meshheading:12904474-Chromosomes, Artificial, Bacterial, pubmed-meshheading:12904474-Gene Expression, pubmed-meshheading:12904474-Green Fluorescent Proteins, pubmed-meshheading:12904474-Hypothalamus, pubmed-meshheading:12904474-Ligands, pubmed-meshheading:12904474-Luminescent Proteins, pubmed-meshheading:12904474-Medulla Oblongata, pubmed-meshheading:12904474-Mice, pubmed-meshheading:12904474-Mice, Transgenic, pubmed-meshheading:12904474-Neurons, pubmed-meshheading:12904474-Paraventricular Hypothalamic Nucleus, pubmed-meshheading:12904474-Patch-Clamp Techniques, pubmed-meshheading:12904474-Promoter Regions, Genetic, pubmed-meshheading:12904474-RNA, Messenger, pubmed-meshheading:12904474-Receptor, Melanocortin, Type 4, pubmed-meshheading:12904474-Receptors, Corticotropin, pubmed-meshheading:12904474-Vagus Nerve
pubmed:year
2003
pubmed:articleTitle
Transgenic mice expressing green fluorescent protein under the control of the melanocortin-4 receptor promoter.
pubmed:affiliation
Laboratory of Molecular Genetics, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.