Linked Life Data

12903175

Source:http://linkedlifedata.com/resource/pubmed/id/12903175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-8-7
pubmed:abstractText
Various four-base codons had been developed for introduction of nonnatural amino acids into proteins in an E. coli in vitro translation system. Here, the four-base codon mediated incorporation of nonnatural amino acids was investigated in a rabbit reticulocyte lysate. Mutated streptavidin mRNAs containing four-base codons were prepared and expressed in a rabbit reticulocyte lysate in the presence of tRNAs containing corresponding four-base anticodons. Western blot analysis indicates that various four-base codons are efficiently decoded by the corresponding tRNAs in the rabbit in vitro translation system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-2
pubmed:dateRevised
2006-11-30
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Nonnatural mutagenesis in E. coli and rabbit reticulocyte lysates by using four-base codons.
pubmed:affiliation
Department of Bioscience and Biotechnology, Okayama University, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article