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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2003-8-20
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pubmed:abstractText |
Type-II topoisomerases are responsible for untangling DNA during replication by removing supercoiled and interlinked DNA structures. Using a single-molecule micromanipulation setup, we follow the real-time decatenation of two mechanically braided DNA molecules by Drosophila melanogaster topoisomerase (Topo) II and Escherichia coli Topo IV. Although Topo II relaxes left-handed (L) and right-handed (R-) braids similarly at a rate of approximately 2.9 s-1, Topo IV has a marked preference for L-braids, which it relaxes completely and processively at a rate of approximately 2.4 s-1. However, Topo IV can unlink R-braids at about half that rate when they supercoil to form L-plectonemes. These results imply that the preferred substrate for unlinking by Topo IV has the symmetry of an L-crossing and shed new light on the decatenation of daughter strands during DNA replication, which are usually assumed to be linked in an R-braid.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-10786800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-11090135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-11459956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-12042765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-12509418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-1330320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-1330327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-1439819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-2203751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-2359128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-6244895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-6323018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-7590259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8079175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8506378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8596951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8702938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8744294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-8940171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9078364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9235892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-94144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9545060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9724746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9748489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9753328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9794033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902541-9794753
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9820-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12902541-Animals,
pubmed-meshheading:12902541-DNA,
pubmed-meshheading:12902541-DNA, Superhelical,
pubmed-meshheading:12902541-DNA Topoisomerase IV,
pubmed-meshheading:12902541-DNA Topoisomerases, Type II,
pubmed-meshheading:12902541-Drosophila melanogaster,
pubmed-meshheading:12902541-Escherichia coli,
pubmed-meshheading:12902541-Models, Molecular,
pubmed-meshheading:12902541-Nucleic Acid Conformation,
pubmed-meshheading:12902541-Prokaryotic Cells,
pubmed-meshheading:12902541-Stereoisomerism
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pubmed:year |
2003
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pubmed:articleTitle |
Single-molecule study of DNA unlinking by eukaryotic and prokaryotic type-II topoisomerases.
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pubmed:affiliation |
Laboratoire de Physique Statistique, Ecole Normale Supérieure, Unité Mixte de Recherche 8550 Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France. gilles.charvin@lps.ens.fr
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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