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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-8-6
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pubmed:abstractText |
The objective of these studies was to examine collagen-induced arthritis (CIA) in C57BL/6 mice transgenic for the rodent complement regulatory protein complement receptor 1-related gene/protein y (Crry) (Crry-Tg), a C3 convertase inhibitor. The scores for clinical disease activity and for histological damage in the joints were both significantly decreased in Crry-Tg mice in comparison to wild-type (WT) littermates. The production of both IgG1 and IgG2a anti-collagen Abs was reduced in the Crry-Tg mice, although spleen cell proliferation in response to collagen type II was not altered. The production of IFN-gamma, TNF-alpha, and IL-1beta by LPS-stimulated spleen cells was decreased, and IL-10 was increased, in cells from Crry-Tg mice in comparison to WT. The steady-state mRNA levels for IFN-gamma, TNF-alpha, and IL-1beta were all decreased in the joints of Crry-Tg mice in comparison to WT. The synovium from Crry-Tg mice without CIA contained the mRNA for the Crry transgene, by RT-PCR, and the synovium from transgenic mice with CIA exhibited little deposition of C3 protein by immunohistological analysis. These results suggest that suppression of CIA in Crry-Tg mice may be due to enhanced synthesis of Crry locally in the joint with decreased production of proinflammatory cytokines.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Complement Inactivator Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Crry protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Complement,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Complement 3b
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2109-15
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12902517-Animals,
pubmed-meshheading:12902517-Arthritis, Experimental,
pubmed-meshheading:12902517-Autoantibodies,
pubmed-meshheading:12902517-Cattle,
pubmed-meshheading:12902517-Collagen Type II,
pubmed-meshheading:12902517-Complement Inactivator Proteins,
pubmed-meshheading:12902517-Cytokines,
pubmed-meshheading:12902517-Female,
pubmed-meshheading:12902517-Hindlimb,
pubmed-meshheading:12902517-Immunoglobulin G,
pubmed-meshheading:12902517-Immunohistochemistry,
pubmed-meshheading:12902517-Injections, Intradermal,
pubmed-meshheading:12902517-Lymph Nodes,
pubmed-meshheading:12902517-Lymphocyte Activation,
pubmed-meshheading:12902517-Lymphocyte Subsets,
pubmed-meshheading:12902517-Male,
pubmed-meshheading:12902517-Mice,
pubmed-meshheading:12902517-Mice, Inbred C57BL,
pubmed-meshheading:12902517-Mice, Transgenic,
pubmed-meshheading:12902517-RNA, Messenger,
pubmed-meshheading:12902517-Receptors, Complement,
pubmed-meshheading:12902517-Receptors, Complement 3b,
pubmed-meshheading:12902517-Spleen
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pubmed:year |
2003
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pubmed:articleTitle |
Prevention of collagen-induced arthritis in mice transgenic for the complement inhibitor complement receptor 1-related gene/protein y.
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pubmed:affiliation |
Division of Rheumatology, University of Colorado Health Sciences Center, Denver, CO 80262. BolderPATH, Boulder, CO 80309, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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