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rdf:type |
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lifeskim:mentions |
umls-concept:C0007745,
umls-concept:C0018270,
umls-concept:C0027740,
umls-concept:C0030685,
umls-concept:C0054493,
umls-concept:C0220839,
umls-concept:C0391871,
umls-concept:C0597357,
umls-concept:C0680255,
umls-concept:C0682702,
umls-concept:C1283071,
umls-concept:C1335268,
umls-concept:C1963578
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pubmed:issue |
42
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pubmed:dateCreated |
2003-10-13
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pubmed:abstractText |
Very little is known about the contribution of a low affinity neurotrophin receptor, p75, to neurotransmitter release. Here we show that nerve growth factor (NGF) induced a rapid release of glutamate and an increase of Ca2+ in cerebellar neurons through a p75-dependent pathway. The NGF-induced release occurred even in the presence of the Trk inhibitor K252a. The release caused by NGF but not brain-derived neurotrophic factor was enhanced in neurons overexpressing p75. Further, after transfection of p75-small interfering RNA, which down-regulated the endogenous p75 expression, the NGF-induced release was inhibited, suggesting that the NGF-induced glutamate release was through p75. We found that the NGF-increased Ca2+ was derived from the ryanodine-sensitive Ca2+ receptor and that the NGF-increased Ca2+ was essential for the NGF-induced glutamate release. Furthermore, scyphostatin, a sphingomyelinase inhibitor, blocked the NGF-dependent Ca2+ increase and glutamate release, suggesting that a ceramide produced by sphingomyelinase was required for the NGF-stimulated Ca2+ increase and glutamate release. This action of NGF only occurred in developing neurons whereas the brain-derived neurotrophic factor-mediated Ca2+ increase and glutamate release was observed at the mature neuronal stage. Thus, we demonstrate that NGF-mediated neurotransmitter release via the p75-dependent pathway has an important role in developing neurons.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane...,
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Ceramides,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine Receptor Calcium Release...,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingomyelin Phosphodiesterase,
http://linkedlifedata.com/resource/pubmed/chemical/scyphostatin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:ArakiToshiyukiT,
pubmed-author:IshimotoTetsuyaT,
pubmed-author:KuboTakekazuT,
pubmed-author:NakayamaHitoshiH,
pubmed-author:NaraFutoshiF,
pubmed-author:NumakawaTadahiroT,
pubmed-author:NumakawaYumikoY,
pubmed-author:OguraAkihikoA,
pubmed-author:SuzukiShingoS,
pubmed-author:TaguchiTakahisaT,
pubmed-author:YokomakuDaisakuD
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41259-69
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12902347-Adenoviridae,
pubmed-meshheading:12902347-Amides,
pubmed-meshheading:12902347-Amino Acids,
pubmed-meshheading:12902347-Animals,
pubmed-meshheading:12902347-Calcium,
pubmed-meshheading:12902347-Cells, Cultured,
pubmed-meshheading:12902347-Ceramides,
pubmed-meshheading:12902347-Cerebellum,
pubmed-meshheading:12902347-Chelating Agents,
pubmed-meshheading:12902347-Dose-Response Relationship, Drug,
pubmed-meshheading:12902347-Down-Regulation,
pubmed-meshheading:12902347-Egtazic Acid,
pubmed-meshheading:12902347-Enzyme Activation,
pubmed-meshheading:12902347-Glutamic Acid,
pubmed-meshheading:12902347-Immunoblotting,
pubmed-meshheading:12902347-Immunohistochemistry,
pubmed-meshheading:12902347-Magnesium,
pubmed-meshheading:12902347-Nerve Growth Factor,
pubmed-meshheading:12902347-Neurons,
pubmed-meshheading:12902347-Pyrones,
pubmed-meshheading:12902347-Rats,
pubmed-meshheading:12902347-Receptor, Nerve Growth Factor,
pubmed-meshheading:12902347-Receptors, Nerve Growth Factor,
pubmed-meshheading:12902347-Ryanodine Receptor Calcium Release Channel,
pubmed-meshheading:12902347-Sphingomyelin Phosphodiesterase,
pubmed-meshheading:12902347-Time Factors,
pubmed-meshheading:12902347-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Nerve growth factor-induced glutamate release is via p75 receptor, ceramide, and Ca(2+) from ryanodine receptor in developing cerebellar neurons.
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pubmed:affiliation |
Neuronics R G Special Division for Human Life Technology National Institute of Advanced Industrial Science and Technology (AIST) Midorigaoka, Ikeda, Osaka 563-8577, Japan. numakawa@ncnp.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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