Source:http://linkedlifedata.com/resource/pubmed/id/12900547
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-4
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| pubmed:dateCreated |
2003-8-5
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| pubmed:abstractText |
In female mammals, one of the two X chromosomes is inactivated to compensate for the difference in dosage of X-linked genes between males and females. X inactivation involves sequential alterations to the chromatin that ultimately lead to the transcriptional repression of genes on the X chromosome. Here, histone methylation and acetylation along X-linked genes are investigated by chromatin immunoprecipitation (ChIP) of adult fibroblast cell lines. At PGK1 and HPRT, chromatin on the active X chromosome reveals H3 lysine 4 methylation and acetylation of histones H3 and H4. These modifications are absent on the repressed allele, which is marked by H3 lysine 9 methylation. On the expressed allele of XIST (on the inactive X chromosome), we found that H3 acetylation is confined to the promoter, whereas H3 lysine 4 methylation and H4 acetylation are present along the entire gene. On the repressed XIST allele, in contrast, the promoter and gene exhibit H3 lysine 9 methylation. At only 1.5 kb upstream of the XIST gene, chromatin on the inactive X chromosome has strongly reduced levels of H4 acetylation and is marked by both H3 lysine 9 and H3 lysine 4 methylation. These data demonstrate that patterns of histone methylation and acetylation are distinct along and upstream of XIST and suggest that the inactive X chromatin configuration occurs at a region close to the 5' end of the gene.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxanthine...,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoglycerate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tsix transcript, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/X (inactive)-specific transcript...
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1424-859X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2003 S. Karger AG, Basel
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| pubmed:issnType |
Electronic
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| pubmed:volume |
99
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
66-74
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:12900547-Acetylation,
pubmed-meshheading:12900547-Alleles,
pubmed-meshheading:12900547-Animals,
pubmed-meshheading:12900547-Cell Line,
pubmed-meshheading:12900547-DNA Methylation,
pubmed-meshheading:12900547-Dosage Compensation, Genetic,
pubmed-meshheading:12900547-Female,
pubmed-meshheading:12900547-Gene Expression Regulation,
pubmed-meshheading:12900547-Genetic Linkage,
pubmed-meshheading:12900547-Histones,
pubmed-meshheading:12900547-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:12900547-Lysine,
pubmed-meshheading:12900547-Male,
pubmed-meshheading:12900547-Methylation,
pubmed-meshheading:12900547-Mice,
pubmed-meshheading:12900547-Phosphoglycerate Kinase,
pubmed-meshheading:12900547-RNA, Untranslated,
pubmed-meshheading:12900547-Transcription Factors,
pubmed-meshheading:12900547-X Chromosome
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| pubmed:year |
2002
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| pubmed:articleTitle |
Differential patterns of histone methylation and acetylation distinguish active and repressed alleles at X-linked genes.
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| pubmed:affiliation |
Institute of Molecular Genetics, CNRS, Montpellier, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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