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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2003-8-4
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pubmed:abstractText |
Site-directed mutagenesis was employed to create lesions in fimI, a gene of uncertain function located in the chromosomal gene cluster (fim) involved in Escherichia coli type 1 pilus biosynthesis. Chromosomal fimI mutations produced a piliation-negative phenotype. Complementation analysis indicated that a fimI'-kan insertion mutation and a fimI frameshift mutation produced polarity-like effects not seen with an in-frame fimI deletion mutation. Minicell analysis associated fimI with a 16.4-kDa noncytoplasmic protein product (FimI). We conclude that FimI has a required role in normal pilus biosynthesis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-11251843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-11395476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-12180926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-1355769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-1363735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-1676398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-1971261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-2172217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-2858471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-2879830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-2882856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-3930469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-4605400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-5318403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-6131870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-6146599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-6147250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-6195290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-7892228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-8097517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-8288102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-8635748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-9278503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12897022-9973330
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5007-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12897022-Adhesins, Escherichia coli,
pubmed-meshheading:12897022-Alleles,
pubmed-meshheading:12897022-Escherichia coli,
pubmed-meshheading:12897022-Fimbriae, Bacterial,
pubmed-meshheading:12897022-Fimbriae Proteins,
pubmed-meshheading:12897022-Frameshift Mutation,
pubmed-meshheading:12897022-Genetic Complementation Test,
pubmed-meshheading:12897022-Molecular Sequence Data,
pubmed-meshheading:12897022-Mutagenesis, Insertional,
pubmed-meshheading:12897022-Mutagenesis, Site-Directed
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pubmed:year |
2003
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pubmed:articleTitle |
The Product of the fimI gene is necessary for Escherichia coli type 1 pilus biosynthesis.
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pubmed:affiliation |
College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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