Source:http://linkedlifedata.com/resource/pubmed/id/12893847
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-9-22
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pubmed:abstractText |
Histidine decarboxylase (HDC) represents the sole enzyme that produces histamine in the body. The present work investigated the role of endogenous histamine in carbachol- and gastrin-induced gastric acid secretion with HDC-knockout (HDC-/-) mice. Acid secretion was measured in either mice subjected to acute fistula production under urethane anesthesia or conscious mice that had previously undergone pylorus ligation. In wild-type mice, carbachol and gastrin significantly stimulated acid secretion, increasing gastric mucosal histamine. In contrast, in HDC-/- mice, carbachol and gastrin had little impact when either delivered alone or together. Nonetheless, the two agents achieved a synergistic effect when delivered together with exogenous histamine, stimulating acid secretion in HDC-/- mice. Such synergism was abolished by the histamine H2-receptor antagonist famotidine. cAMP involvement in acid secretion was also examined with theophylline, a phosphodiesterase inhibitor, and forskolin, an adenylate cyclase activator. In wild-type mice, theophylline significantly increased acid secretion, enhancing carbachol- and gastrin-stimulated acid secretion. In contrast, in HDC-/- mice, theophylline failed to exert an effect on basal acid secretion, as well as carbachol- and gastrin-stimulated acid secretion. Although forskolin interacted with carbachol, allowing acid secretion in HDC-/- mice, similar results were not achieved with gastrin. Such results suggest that 1) histamine is essential for carbachol- and gastrin-stimulated gastric acid secretion in mice; and 2) histamine-induced cAMP production contributes to the in vivo response to carbachol or gastrin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Gastrins,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Theophylline
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3565
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
307
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:12893847-Animals,
pubmed-meshheading:12893847-Carbachol,
pubmed-meshheading:12893847-Female,
pubmed-meshheading:12893847-Forskolin,
pubmed-meshheading:12893847-Gastric Acid,
pubmed-meshheading:12893847-Gastrins,
pubmed-meshheading:12893847-Histamine,
pubmed-meshheading:12893847-Histidine Decarboxylase,
pubmed-meshheading:12893847-Hydrogen-Ion Concentration,
pubmed-meshheading:12893847-Male,
pubmed-meshheading:12893847-Mice,
pubmed-meshheading:12893847-Mice, Inbred ICR,
pubmed-meshheading:12893847-Mice, Knockout,
pubmed-meshheading:12893847-Theophylline
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pubmed:year |
2003
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pubmed:articleTitle |
Crucial role of histamine for regulation of gastric acid secretion ascertained by histidine decarboxylase-knockout mice.
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pubmed:affiliation |
Department of Applied Pharmacology, Kyoto Pharmaceutical University, Misasagi, Yamashina, Japan.
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pubmed:publicationType |
Journal Article
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