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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2003-7-31
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pubmed:abstractText |
An open reading frame, rub52, has been identified as a gene encoding thymidine diphospho-glucose 2,3-dehydratase by sequence analysis of the rubradirin biosynthetic gene cluster of Streptomyces achromogenes var. nibradiris NRRL3061. The gene codes for a protein consisting of 458 amino acids with calculated molecular mass of 50862 Da. The gene was amplified and heterologously expressed in Escherichia coli as a soluble His-tagged fusion protein. C-2 deoxygenation functionality of thymidine diphospho-4-keto-6-deoxyglucose was assigned to the rub52 gene product from in vitro enzyme assay.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
909-15
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pubmed:dateRevised |
2009-11-3
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pubmed:meshHeading |
pubmed-meshheading:12889805-Amino Acid Sequence,
pubmed-meshheading:12889805-Cloning, Molecular,
pubmed-meshheading:12889805-Escherichia coli,
pubmed-meshheading:12889805-Gene Expression Profiling,
pubmed-meshheading:12889805-Gene Expression Regulation, Bacterial,
pubmed-meshheading:12889805-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:12889805-Glycosides,
pubmed-meshheading:12889805-Hydro-Lyases,
pubmed-meshheading:12889805-Molecular Sequence Data,
pubmed-meshheading:12889805-Naphthoquinones,
pubmed-meshheading:12889805-Recombinant Fusion Proteins,
pubmed-meshheading:12889805-Sequence Alignment,
pubmed-meshheading:12889805-Sequence Analysis, Protein,
pubmed-meshheading:12889805-Streptomyces
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pubmed:year |
2003
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pubmed:articleTitle |
Functional identification of rub52 gene involved in the biosynthesis of rubradirin.
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pubmed:affiliation |
Institute of Biomolecule Reconstruction, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam 336-708, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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