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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-7-30
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pubmed:abstractText |
Cellular signaling proteins such as metabotropic glutamate receptors, Shank, and different types of ion channels are physically linked by Vesl (VASP/Ena-related gene up-regulated during seizure and LTP)/Homer proteins [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851]. Vesl/Homer proteins have also been implicated in differentiation and physiological adaptation processes [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962; Biochem. Biophys. Res. Commun. 279 (2000) 348]. Here we provide evidence that a Vesl/Homer subtype, Vesl-1L/Homer-1c (V-1L), reduces the function of the intracellular calcium channel ryanodine receptor type 2 (RyR2). In contrast, Vesl-1S/Homer-1a (V-1S) had no effect on RyR2 function but reversed the effects of V-1L. In live cells, in calcium release studies and in single-channel electrophysiological recordings of RyR2, V-1L reduced RyR2 activity. Important physiological functions and pharmacological properties of RyR2 are preserved in the presence of V-1L. Our findings demonstrate that a protein-protein interaction between V-1L and RyR2 is not only necessary for organizing the structure of intracellular calcium signaling proteins [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23(2000)80; J. Cell Sci. 113 (2000) 1851; Nat Neurosci. 4 (2001) 499; Nature 411 (2001) 962; Biochem. Biophys. Res. Commun. 279 (2000) 348; Nature 386 (1997) 284], but that V-1L also directly regulates RyR2 channel activity by changing its biophysical properties. Thereby it may control cellular calcium homeostasis. These observations suggest a novel mechanism for the regulation of RyR2 and calcium-dependent cellular functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane...,
http://linkedlifedata.com/resource/pubmed/chemical/Aniline Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic ADP-Ribose,
http://linkedlifedata.com/resource/pubmed/chemical/Dantrolene,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Fluo-3,
http://linkedlifedata.com/resource/pubmed/chemical/Homer protein,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine,
http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine Receptor Calcium Release...,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthenes
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0143-4160
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12887973-Aniline Compounds,
pubmed-meshheading:12887973-Animals,
pubmed-meshheading:12887973-Binding Sites,
pubmed-meshheading:12887973-Blotting, Western,
pubmed-meshheading:12887973-CHO Cells,
pubmed-meshheading:12887973-Caffeine,
pubmed-meshheading:12887973-Calcium,
pubmed-meshheading:12887973-Calcium Signaling,
pubmed-meshheading:12887973-Carrier Proteins,
pubmed-meshheading:12887973-Cricetinae,
pubmed-meshheading:12887973-Cyclic ADP-Ribose,
pubmed-meshheading:12887973-Dantrolene,
pubmed-meshheading:12887973-Dose-Response Relationship, Drug,
pubmed-meshheading:12887973-Egtazic Acid,
pubmed-meshheading:12887973-Electrophysiology,
pubmed-meshheading:12887973-Microscopy, Fluorescence,
pubmed-meshheading:12887973-Myocardium,
pubmed-meshheading:12887973-Neuropeptides,
pubmed-meshheading:12887973-Protein Binding,
pubmed-meshheading:12887973-Protein Isoforms,
pubmed-meshheading:12887973-Rats,
pubmed-meshheading:12887973-Ryanodine,
pubmed-meshheading:12887973-Ryanodine Receptor Calcium Release Channel,
pubmed-meshheading:12887973-Sarcoplasmic Reticulum,
pubmed-meshheading:12887973-Transfection,
pubmed-meshheading:12887973-Xanthenes
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pubmed:year |
2003
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pubmed:articleTitle |
Vesl/Homer proteins regulate ryanodine receptor type 2 function and intracellular calcium signaling.
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pubmed:affiliation |
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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