Linked Life Data

12887316

Source:http://linkedlifedata.com/resource/pubmed/id/12887316

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
2003-7-30
pubmed:abstractText
Exocytotic release of neuropeptides and hormones is generally believed to involve the complete merger of the secretory vesicle with the plasma membrane. However, recent data have suggested that 'kiss-and-run' mechanisms may also play a role. To analyse secretory events in neuroendocrine beta-cells, we imaged chimaeric reporters targeted to either the vesicle membrane [chimaeras of synaptobrevin-2 and pH-sensitive green fluorescent protein (synapto.pHluorin) or of phogrin (phosphatase on the granule of insulinoma) and enhanced green fluorescent protein (EGFP) (phogrin.EGFP)] or the lumen [neuropeptide Y (NPY).pH-insensitive yellow fluorescent protein (Venus)] by evanescent wave microscopy. Unexpectedly, the frequency of NPY.Venus release events was only 17-27% of that of vesicle fusion reported with synapto.pHluorin, but not phogrin.EGFP, indicating that exocytosis of cargo peptides that is likely to require complete collapse of the vesicle into the plasma membrane is relatively rare. However, both the frequency and the kinetics of NPY.Venus release were modulated by stimulus strength or by overexpression of synaptotagmin IV, demonstrating the plasticity of 'kiss-and-run' fusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0300-5127
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
833-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Insulin secretion by 'kiss-and-run' exocytosis in clonal pancreatic islet beta-cells.
pubmed:affiliation
Henry Wellcome Laboratories for Integrated Cell Signalling and Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK.
pubmed:publicationType
Journal Article, Review