Source:http://linkedlifedata.com/resource/pubmed/id/12886245
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2003-7-29
|
| pubmed:abstractText |
Detection of minimal residual disease (MRD), using immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements as clone-specific targets, represents the most recent development in diagnosis and treatment of acute lymphoblastic leukaemia (ALL). Nevertheless, risk of false-negative results, due to secondary or ongoing rearrangements of Ig/TCR genes during the disease course, might hamper MRD detection. Therefore, to gain extensive information on clonal stability, we performed PCR-GeneScan analysis of Ig/TCR gene rearrangements at diagnosis and subsequent relapse in bone marrow samples from 53 childhood precursor-B-ALL patients. In addition, sequencing analysis of junctional regions at diagnosis and relapse provided a detailed insight in the stability and changes of Ig/TCR gene rearrangements during the disease course. At least one stable clonal Ig/TCR target was found in 94% of patients. In three patients complete differences in Ig/TCR rearrangements between diagnosis and relapse were observed, suggesting relapse with a new clone. At relapse, 71% of diagnostic clonal PCR targets was conserved. Since the comparison of Ig/TCR gene rearrangements at diagnosis and relapse in our precursor-B-ALL patients did not show significant difference in the stability of different clonal PCR targets (IGH, 70%; IGK, 71%; TCRD, 67%; TCRG, 75%), we conclude that there is no 'preferential' clone-specific target for MRD monitoring.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
0887-6924
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
17
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1573-82
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12886245-Adolescent,
pubmed-meshheading:12886245-Child,
pubmed-meshheading:12886245-Child, Preschool,
pubmed-meshheading:12886245-Clone Cells,
pubmed-meshheading:12886245-Female,
pubmed-meshheading:12886245-Follow-Up Studies,
pubmed-meshheading:12886245-Gene Rearrangement, B-Lymphocyte,
pubmed-meshheading:12886245-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:12886245-Genes, Immunoglobulin,
pubmed-meshheading:12886245-Humans,
pubmed-meshheading:12886245-Infant,
pubmed-meshheading:12886245-Male,
pubmed-meshheading:12886245-Neoplasm, Residual,
pubmed-meshheading:12886245-Polymerase Chain Reaction,
pubmed-meshheading:12886245-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:12886245-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:12886245-Recurrence,
pubmed-meshheading:12886245-Sequence Analysis, DNA,
pubmed-meshheading:12886245-Time Factors
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Clonality profile in relapsed precursor-B-ALL children by GeneScan and sequencing analyses. Consequences on minimal residual disease monitoring.
|
| pubmed:affiliation |
Laboratorio di Emato Oncologia, Dipartimento di Pediatria, Universita' di Padova, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|