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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-7-29
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pubmed:abstractText |
Mannoproteins, as the main constituents of the outer layer of yeast cell walls, are able to interact with phagocytic cells in an opsonin-independent manner through the mannose receptor (MR) and to induce yeast ingestion by the professional phagocytes. Moreover, the MR also mediates endocytosis of soluble ligands through clathrin-coated pits. Here, we studied some aspects of the interaction between the MR and Candida albicans using murine E-clone macrophages and the consequences on MR trafficking. Using a pull-down assay involving mixture E-clone macrophage detergent lysate with mannosylated Sepharose beads and glutaraldehyde-fixed, heat-killed (HK) C. albicans, we found that binding of solubilized MR to mannosylated particles occurred with characteristics similar to the receptor's cell-surface mannose-binding activity. We then demonstrated that MR expressed on E-clone macrophages contributed to phagocytosis of unopsonized, HK C. albicans and that yeast phagocytosis induced a decrease in MR endocytic activity without concomitant degradation of the receptor in the time lapse studied.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0741-5400
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
206-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12885937-Animals,
pubmed-meshheading:12885937-Candida albicans,
pubmed-meshheading:12885937-Cell Line,
pubmed-meshheading:12885937-Clone Cells,
pubmed-meshheading:12885937-Hot Temperature,
pubmed-meshheading:12885937-Humans,
pubmed-meshheading:12885937-Lectins, C-Type,
pubmed-meshheading:12885937-Macrophage Activation,
pubmed-meshheading:12885937-Macrophages,
pubmed-meshheading:12885937-Mannans,
pubmed-meshheading:12885937-Mannose-Binding Lectins,
pubmed-meshheading:12885937-Mice,
pubmed-meshheading:12885937-Microspheres,
pubmed-meshheading:12885937-Opsonin Proteins,
pubmed-meshheading:12885937-Phagocytosis,
pubmed-meshheading:12885937-Phagosomes,
pubmed-meshheading:12885937-Receptors, Cell Surface,
pubmed-meshheading:12885937-Sepharose,
pubmed-meshheading:12885937-Transferrin
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pubmed:year |
2003
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pubmed:articleTitle |
Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages.
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pubmed:affiliation |
Laboratoire d'Immunologie et de Parasitologie EA 2413, Université Montpellier I, France. UMR CNRS 5539, Université Montpellier II, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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