Linked Life Data

12885908

Source:http://linkedlifedata.com/resource/pubmed/id/12885908

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2003-7-29
pubmed:abstractText
Canine parvovirus (CPV) and feline panleukopenia virus (FPV) capsids bind to the transferrin receptors (TfRs) of their hosts and use these receptors to infect cells. The binding is partially host specific, as FPV binds only to the feline TfR, while CPV binds to both the canine and feline TfRs. The host-specific binding is controlled by a combination of residues within a raised region of the capsid. To define the TfR structures that interact with the virus, we altered the apical domain of the feline or canine TfR or prepared chimeras of these receptors and tested the altered receptors for binding to FPV or CPV capsids. Most changes in the apical domain of the feline TfR did not affect binding, but replacing Leu221 with Ser or Asp prevented receptor binding to either FPV or CPV capsids, while replacing Leu221 with Lys resulted in a receptor that bound only to CPV but not to FPV. Analysis of recombinants of the feline and canine TfRs showed that sequences controlling CPV-specific binding were within the apical domain and that more than one difference between these receptors determined the CPV-specific binding of the canine TfR. Single changes within the canine TfR which removed a single amino acid insertion or which eliminated a glycosylation site gave that receptor the expanded ability to bind to FPV and CPV. In some cases, binding of capsids to mutant receptors did not result in infection, suggesting a structural role for the receptor in cell infection by the viruses.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10189373, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10470084, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10531064, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10638746, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10644365, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10753702, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10756055, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10775624, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-10884355, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11264378, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11435568, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11514193, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11559807, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11560765, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11687498, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11799183, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-11800564, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-12051899, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-12525605, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-12584318, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-1331498, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-1379471, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-1647068, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-1825254, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-1942246, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-2403553, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-2722854, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-3611186, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-4735577, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-5578914, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-7609035, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-7611627, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-7780197, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8207398, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8392729, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8463276, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8631371, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8918534, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-8970738, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-9371580, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-9490719, http://linkedlifedata.com/resource/pubmed/commentcorrection/12885908-9557871
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8915-23
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Residues in the apical domain of the feline and canine transferrin receptors control host-specific binding and cell infection of canine and feline parvoviruses.
pubmed:affiliation
James A. Baker Institute, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.