Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2003-7-29
pubmed:abstractText
This chapter describes the function and development of the ciliary ganglion, the potential of ciliary ganglion neurons as a cell biological tool, and their dissection, dissociation, and culturing. Ciliary ganglion neurons grow unusually rapidly on a laminin-based substratum and develop large, thin calyx terminals in culture in less than 12 h. The two neuronal classes present in the cultures can be identified by size alone. The limited number of ganglia per animal renders this ganglion a poor choice for biochemical studies based on the extraction of cultured cells. However, they are ideally suited for studies based on single-cell observation, particularly investigation of presynaptic mechanisms using fluorescence microscopy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0091-679X
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-50
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Dissection and culturing of chick ciliary ganglion neurons: a system well suited to synaptic study.
pubmed:affiliation
Department of Biochemistry and Molecular Biology and Program in Molecular, Cellular, and Integrative Neurosciences, Colorado State University, Fort Collins, Colorado 80523, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't