| pubmed-article:12883880 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0376358 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0668084 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12883880 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12883880 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12883880 | pubmed:dateCreated | 2003-7-28 | lld:pubmed |
| pubmed-article:12883880 | pubmed:abstractText | Functional protease-activated receptors (PAR) are expressed by a variety of malignant cells. In the present study, RT-PCR assays demonstrated the expression of the thrombin receptor PAR-1 mRNA in human prostate cancer cell lines DU 145, LnCAP, and SV40-immortalized human prostate epithelial cell line PNT1A. In contrast, the additional thrombin receptors PAR-3 and PAR-4 were not detected. PAR-1 protein localized to the cellular surface was detected by flow cytometry in all three cell lines. To demonstrate the functional importance of the PAR-1, the effects of different concentrations of thrombin on cell proliferation kinetics were assessed. The treatment of growth-arrested cells with varying concentrations of thrombin demonstrated dose- and time-dependent effects. At low concentration (<0.5 U/ml), thrombin induced proliferation of all prostate-derived cell lines. Thrombin at higher concentration (1.0 U/ml) initially stimulated PNT1A and LnCAP cells to proliferate (time of thrombin application 24 h and 48 h) followed by inhibited growth when assessed after 72 h of incubation. In contrast, 1.0 U/ml thrombin caused earlier inhibition of DU 145 proliferation starting at 48 h of incubation. Our results suggest that PAR-1 mediates the proliferation-modulating effects of thrombin on prostate cancer cells. | lld:pubmed |
| pubmed-article:12883880 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12883880 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12883880 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12883880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12883880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12883880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12883880 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12883880 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12883880 | pubmed:issn | 0300-5623 | lld:pubmed |
| pubmed-article:12883880 | pubmed:author | pubmed-author:Schuff-Werner... | lld:pubmed |
| pubmed-article:12883880 | pubmed:author | pubmed-author:LiuJianJ | lld:pubmed |
| pubmed-article:12883880 | pubmed:author | pubmed-author:SteinerMichae... | lld:pubmed |
| pubmed-article:12883880 | pubmed:author | pubmed-author:BastianManuel... | lld:pubmed |
| pubmed-article:12883880 | pubmed:author | pubmed-author:KohlscheinPet... | lld:pubmed |
| pubmed-article:12883880 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12883880 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:12883880 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12883880 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12883880 | pubmed:pagination | 163-8 | lld:pubmed |
| pubmed-article:12883880 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
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| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:meshHeading | pubmed-meshheading:12883880... | lld:pubmed |
| pubmed-article:12883880 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12883880 | pubmed:articleTitle | Expression of functional protease-activated receptor 1 in human prostate cancer cell lines. | lld:pubmed |
| pubmed-article:12883880 | pubmed:affiliation | University of Rostock, Medical Faculty, Institute of Clinical Chemistry and Pathobiochemistry, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany. | lld:pubmed |
| pubmed-article:12883880 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12883880 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:2149 | entrezgene:pubmed | pubmed-article:12883880 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12883880 | lld:entrezgene |
| lhgdn:association:47401 | lhgdn:found_in | pubmed-article:12883880 | lld:lhgdn |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12883880 | lld:pubmed |
