Source:http://linkedlifedata.com/resource/pubmed/id/12883880
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2003-7-28
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pubmed:abstractText |
Functional protease-activated receptors (PAR) are expressed by a variety of malignant cells. In the present study, RT-PCR assays demonstrated the expression of the thrombin receptor PAR-1 mRNA in human prostate cancer cell lines DU 145, LnCAP, and SV40-immortalized human prostate epithelial cell line PNT1A. In contrast, the additional thrombin receptors PAR-3 and PAR-4 were not detected. PAR-1 protein localized to the cellular surface was detected by flow cytometry in all three cell lines. To demonstrate the functional importance of the PAR-1, the effects of different concentrations of thrombin on cell proliferation kinetics were assessed. The treatment of growth-arrested cells with varying concentrations of thrombin demonstrated dose- and time-dependent effects. At low concentration (<0.5 U/ml), thrombin induced proliferation of all prostate-derived cell lines. Thrombin at higher concentration (1.0 U/ml) initially stimulated PNT1A and LnCAP cells to proliferate (time of thrombin application 24 h and 48 h) followed by inhibited growth when assessed after 72 h of incubation. In contrast, 1.0 U/ml thrombin caused earlier inhibition of DU 145 proliferation starting at 48 h of incubation. Our results suggest that PAR-1 mediates the proliferation-modulating effects of thrombin on prostate cancer cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0300-5623
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12883880-Cell Division,
pubmed-meshheading:12883880-Cell Line, Tumor,
pubmed-meshheading:12883880-Cell Membrane,
pubmed-meshheading:12883880-Dose-Response Relationship, Drug,
pubmed-meshheading:12883880-Humans,
pubmed-meshheading:12883880-Male,
pubmed-meshheading:12883880-Osmolar Concentration,
pubmed-meshheading:12883880-Prostatic Neoplasms,
pubmed-meshheading:12883880-RNA, Messenger,
pubmed-meshheading:12883880-Receptor, PAR-1,
pubmed-meshheading:12883880-Thrombin,
pubmed-meshheading:12883880-Time Factors,
pubmed-meshheading:12883880-Tissue Distribution
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pubmed:year |
2003
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pubmed:articleTitle |
Expression of functional protease-activated receptor 1 in human prostate cancer cell lines.
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pubmed:affiliation |
University of Rostock, Medical Faculty, Institute of Clinical Chemistry and Pathobiochemistry, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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