Source:http://linkedlifedata.com/resource/pubmed/id/12883535
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
|
| pubmed:dateCreated |
2003-7-28
|
| pubmed:abstractText |
T-lymphocyte-directed gene therapy has potential as a treatment of subjects with immunological disorders. One current limitation of this therapeutic strategy is low gene transfer efficiency, even when complex procedures are used. We report herein that a recombinant Sendai virus vector (SeV) was able to overcome this issue. Using jellyfish enhanced green fluorescent protein gene (EGFP), we found that SeV was able to transduce and express a foreign gene specifically and efficiently in activated murine and human T cells, but not in naive T cells, without centrifugation or reagents including polybrene and protamine sulfate; the present findings were in clear contrast to those demonstrated with the use of retroviruses. The transduction was selective in antigen-activated T cells, while antigen-irrelevant T cells were not transduced, even under bystander activation from specific T-cell responses by antigens ex vivo. Receptor saturation studies suggested a possible mechanism of activated T-cell-specific gene transfer, ie, SeV might attach to naive T cells but might be unable to enter their cytoplasm. We therefore propose that the SeV vector system may prove to be a potentially important alternative in the area of T-cell-directed gene therapy used in the clinical setting.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0969-7128
|
| pubmed:author |
pubmed-author:HasegawaMM,
pubmed-author:HashimotoSS,
pubmed-author:KishiharaKK,
pubmed-author:NagataSS,
pubmed-author:NakagawaKK,
pubmed-author:NakashimaYY,
pubmed-author:OkanoSS,
pubmed-author:OnimaruMM,
pubmed-author:SataSS,
pubmed-author:SueishiKK,
pubmed-author:SugimachiKK,
pubmed-author:TomitaYY,
pubmed-author:YonemitsuYY
|
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1381-91
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12883535-Animals,
pubmed-meshheading:12883535-Cell Line,
pubmed-meshheading:12883535-Female,
pubmed-meshheading:12883535-Gene Expression,
pubmed-meshheading:12883535-Gene Therapy,
pubmed-meshheading:12883535-Genetic Vectors,
pubmed-meshheading:12883535-Green Fluorescent Proteins,
pubmed-meshheading:12883535-Humans,
pubmed-meshheading:12883535-Immunotherapy, Adoptive,
pubmed-meshheading:12883535-Luminescent Proteins,
pubmed-meshheading:12883535-Lymphocyte Activation,
pubmed-meshheading:12883535-Mice,
pubmed-meshheading:12883535-Mice, Inbred BALB C,
pubmed-meshheading:12883535-Mice, Inbred C57BL,
pubmed-meshheading:12883535-Mice, Transgenic,
pubmed-meshheading:12883535-Receptors, Antigen, T-Cell,
pubmed-meshheading:12883535-Sendai virus,
pubmed-meshheading:12883535-T-Lymphocytes,
pubmed-meshheading:12883535-Time Factors
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Recombinant Sendai virus vectors for activated T lymphocytes.
|
| pubmed:affiliation |
Division of Pathophygiological and Experimental Pathology, Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|