Source:http://linkedlifedata.com/resource/pubmed/id/12883492
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2003-7-28
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| pubmed:abstractText |
The cellular retinol-binding protein-1 (CRBP-1) plays a key role in the esterification and intercellular transfer of retinol. By in situ hybridization, immunohistochemistry, and confocal laser scanning microscopy (CLSM), we show that, in normal liver, CRBP-1 is strongly expressed in the cytoplasm of hepatic stellate cells (HSCs) and myofibroblasts (MFs) with only low CRBP-1 levels in hepatocytes. By contrast, in 196 hepatocellular carcinoma (HCC) specimens CRBP-1 expression in MFs was down-regulated in 83%. Patients with high CRBP-1 expression in MFs had a significantly higher 2-year survival as compared with patients with low CRBP-1 expression (52% vs. 29%, respectively; P =.034). An aberrant nuclear CRBP-1 accumulation resulting from cytoplasmic invagination was found in 29% of HCCs. Nuclear CRBP-1 staining correlated positively with a favorable tumor stage (Okuda stage I; P =.01) and negatively with the Ki-67(+) proliferation fraction (PF). A Ki-67(+) PF of > or =10% was associated with a lower 2-year survival probability as compared with patients with a Ki-67(+) PF of <10% (12% vs. 40%, respectively; P =.015). Prognosis did not correlate with the nuclear beta-catenin expression. There was, however, a close correlation between nuclear CRBP-1 inclusions and nuclear beta-catenin staining in HCCs (P =.008), suggesting a cross talk between CRBP-1 and the Wnt/wingless signal transduction pathway. In conclusion, our findings demonstrate that CRBP-1 detection may be useful for the discrimination between nonneoplastic and neoplastic liver cells and suggest that modulation of CRBP-1 expression in HCCs contributes to tumor growth and progression via retinoid-mediated signaling and disruption of cellular vitamin A homeostasis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ki-67 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoids,
http://linkedlifedata.com/resource/pubmed/chemical/Retinol-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinol-Binding Proteins, Cellular,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin A,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0270-9139
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
38
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
470-80
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12883492-Carcinoma, Hepatocellular,
pubmed-meshheading:12883492-Cytoplasm,
pubmed-meshheading:12883492-Cytoskeletal Proteins,
pubmed-meshheading:12883492-Esterification,
pubmed-meshheading:12883492-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12883492-Humans,
pubmed-meshheading:12883492-Ki-67 Antigen,
pubmed-meshheading:12883492-Liver,
pubmed-meshheading:12883492-Liver Cirrhosis,
pubmed-meshheading:12883492-Liver Neoplasms,
pubmed-meshheading:12883492-Proto-Oncogene Proteins,
pubmed-meshheading:12883492-Retinoids,
pubmed-meshheading:12883492-Retinol-Binding Proteins,
pubmed-meshheading:12883492-Retinol-Binding Proteins, Cellular,
pubmed-meshheading:12883492-Signal Transduction,
pubmed-meshheading:12883492-Survival Rate,
pubmed-meshheading:12883492-Trans-Activators,
pubmed-meshheading:12883492-Vitamin A,
pubmed-meshheading:12883492-Wnt Proteins,
pubmed-meshheading:12883492-Zebrafish Proteins,
pubmed-meshheading:12883492-beta Catenin
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| pubmed:year |
2003
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| pubmed:articleTitle |
Cellular retinol-binding protein-1 in hepatocellular carcinoma correlates with beta-catenin, Ki-67 index, and patient survival.
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| pubmed:affiliation |
Institute of Pathology, University of Freiburg, Freiburg, Germany. ahsg@ukl.uni-freiburg.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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