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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2003-7-25
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pubmed:abstractText |
We previously developed a real-time quantitative RT-PCR technique to detect breast carcinoma cells in peripheral blood (PB). The aim of the current study was to improve cytokeratin 19 (CK19) quantification using plasmid dilutions of cloned PCR fragments to obtain a more reliable and reproducible quantification of CK19 transcripts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0923-7534
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pubmed:author |
pubmed-author:AertsJ L EJL,
pubmed-author:AnnéJJ,
pubmed-author:ChristiaensM RMR,
pubmed-author:GaafarRR,
pubmed-author:IsmailM SaadMS,
pubmed-author:KhaledH MHM,
pubmed-author:OmarSS,
pubmed-author:ParidaensRR,
pubmed-author:ShakankiryNN,
pubmed-author:Van MellaertLL,
pubmed-author:Van OosteromA TAT,
pubmed-author:VandekerckhovePP,
pubmed-author:WynendaeleWW
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1241-5
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12881386-Breast Neoplasms,
pubmed-meshheading:12881386-Case-Control Studies,
pubmed-meshheading:12881386-Confidence Intervals,
pubmed-meshheading:12881386-Female,
pubmed-meshheading:12881386-Humans,
pubmed-meshheading:12881386-Indicator Dilution Techniques,
pubmed-meshheading:12881386-Keratins,
pubmed-meshheading:12881386-Neoplasm Invasiveness,
pubmed-meshheading:12881386-Neoplastic Cells, Circulating,
pubmed-meshheading:12881386-Plasmids,
pubmed-meshheading:12881386-Probability,
pubmed-meshheading:12881386-Prospective Studies,
pubmed-meshheading:12881386-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12881386-Sensitivity and Specificity,
pubmed-meshheading:12881386-Statistics, Nonparametric,
pubmed-meshheading:12881386-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Real-time quantitative RT-PCR and detection of tumour cell dissemination in breast cancer patients: plasmid versus cell line dilutions.
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pubmed:affiliation |
Department of Oncology, UZ Gasthuisberg, Leuven, Belgium.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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