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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-7-25
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pubmed:abstractText |
Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1083-3668
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2003 Society of Photo-Optical Instrumentation Engineers.
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
381-90
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12880343-Adenylate Kinase,
pubmed-meshheading:12880343-Algorithms,
pubmed-meshheading:12880343-Animals,
pubmed-meshheading:12880343-Caenorhabditis elegans,
pubmed-meshheading:12880343-Caenorhabditis elegans Proteins,
pubmed-meshheading:12880343-Cell Line, Tumor,
pubmed-meshheading:12880343-Cells, Cultured,
pubmed-meshheading:12880343-Computer Simulation,
pubmed-meshheading:12880343-Fluorescein,
pubmed-meshheading:12880343-Fourier Analysis,
pubmed-meshheading:12880343-Humans,
pubmed-meshheading:12880343-Image Enhancement,
pubmed-meshheading:12880343-Integrin beta Chains,
pubmed-meshheading:12880343-Laryngeal Neoplasms,
pubmed-meshheading:12880343-Microscopy, Confocal,
pubmed-meshheading:12880343-Microscopy, Fluorescence, Multiphoton,
pubmed-meshheading:12880343-Muscle, Skeletal,
pubmed-meshheading:12880343-Recombinant Fusion Proteins,
pubmed-meshheading:12880343-Reproducibility of Results,
pubmed-meshheading:12880343-Sensitivity and Specificity,
pubmed-meshheading:12880343-Time Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods.
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pubmed:affiliation |
University of Illinois at Urbana-Champaign, Laboratory for Fluorescence Dynamics, 1110 West Green Street Urbana, Illinois 61801, USA. Enrico@scs.uiuc.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Validation Studies
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