Linked Life Data

12878703

Source:http://linkedlifedata.com/resource/pubmed/id/12878703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2003-7-24
pubmed:abstractText
The maintenance of long-term potentiation (LTP) depends on alteration of gene transcription. By screening a subtracted cDNA library that is enriched in upregulated transcripts in rat hippocampus 3 hr after Schaffer-CA1 LTP induction in vivo, we identified a neural growth-associated protein SCG10 (superior cervical ganglia clone 10) gene. The semiquantitative reverse transcription-PCR and Northern blot experiments confirmed that SCG10 mRNA levels were elevated in tetanized rat hippocampi compared with those of sham controls that received only low-frequency stimulation. Both 1 and 2 kb forms of SCG10 mRNAs contributed to the increased expression. Using a riboprobe with a sequence specific to the 3'-untranslated region of rat SCG10 mRNA, in situ hybridization further revealed a significant increase of the SCG10 mRNA 2 kb form in the ipsilateral CA3 and CA1 regions of LTP animals. In addition, we systemically injected the competitive NMDA receptor antagonist d,l-3[(+/-)-2-carboxypiperazine-4-yl]-propyl-1-phosphonic acid (CPP) to determine whether the alteration of SCG10 expression depends on NMDA receptor activation or tetanus alone. Administration of CPP 1 hr before tetanus completely blocked LTP induction and the increase of SCG10 mRNA levels. Thus, these results suggest that the transcription of SCG10 in vivo is regulated by long-lasting synaptic activity and may contribute to the maintenance of long-term synaptic plasticity via a presynaptic remodeling mechanism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6617-26
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12878703-Animals, pubmed-meshheading:12878703-Blotting, Northern, pubmed-meshheading:12878703-Carrier Proteins, pubmed-meshheading:12878703-Electric Stimulation, pubmed-meshheading:12878703-Electrodes, Implanted, pubmed-meshheading:12878703-Excitatory Amino Acid Antagonists, pubmed-meshheading:12878703-Gene Expression Profiling, pubmed-meshheading:12878703-Hippocampus, pubmed-meshheading:12878703-In Situ Hybridization, pubmed-meshheading:12878703-Long-Term Potentiation, pubmed-meshheading:12878703-Male, pubmed-meshheading:12878703-Membrane Proteins, pubmed-meshheading:12878703-Nerve Growth Factors, pubmed-meshheading:12878703-Neural Pathways, pubmed-meshheading:12878703-Neuronal Plasticity, pubmed-meshheading:12878703-Piperazines, pubmed-meshheading:12878703-RNA, Messenger, pubmed-meshheading:12878703-Rats, pubmed-meshheading:12878703-Rats, Inbred F344, pubmed-meshheading:12878703-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:12878703-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12878703-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
Identification of upregulated SCG10 mRNA expression associated with late-phase long-term potentiation in the rat hippocampal Schaffer-CA1 pathway in vivo.
pubmed:affiliation
Cajal Neuroscience Institute, Department of Biology, University of Texas, San Antonio, Texas 78249-0662, USA. hpeng@lonestar.utsa.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't