Source:http://linkedlifedata.com/resource/pubmed/id/12875902
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
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| pubmed:dateCreated |
2003-7-23
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| pubmed:abstractText |
It is now recognized that precise patterns of differentially expressed genes ultimately direct a particular cell toward a given lineage. In this study, we compared the expression profiles of cancer-related genes by cDNA microarray analysis during the differentiation of human promyelocytic leukemia HL-60 cells into either monocytes or granulocytes. RNA was isolated at times 0, 6, 12, 24, 36, 48, and 72 h following stimulation of differentiation with all-trans retinoic acid (all-trans RA) or 1,25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)], and hybridized to the microarray gene chips containing 872 genes related to cell-cycles, oncogenes and leukemias. Several genes were commonly or differentially regulated during cell differentiation into either lineage, as demonstrated by both hierarchical and self-organizing map clustering analysis. At 72 h the expression levels of 45 genes were commonly up- or down-regulated at least a twofold in both lineages. Most importantly, 32 genes including alpha-L-fucosidase gene and adducin gamma subunit gene were up- or down-regulated only in all-trans RA-treated HL-60 cells, while 12 genes including interleukin 1beta and hypoxia-inducible factor 1alpha were up- or down-regulated only in 1,25-(OH)(2)D(3)-treated HL-60 cells. The expression of selected genes was confirmed by Northern blot analysis. As expected, some genes identified have not been examined during HL-60 cell differentiation into either lineage. The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0024-3205
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
73
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1705-19
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12875902-Calcitriol,
pubmed-meshheading:12875902-Cell Division,
pubmed-meshheading:12875902-Cell Lineage,
pubmed-meshheading:12875902-Cell Survival,
pubmed-meshheading:12875902-Cell Transformation, Neoplastic,
pubmed-meshheading:12875902-DNA, Neoplasm,
pubmed-meshheading:12875902-Gene Expression Profiling,
pubmed-meshheading:12875902-Gene Expression Regulation, Leukemic,
pubmed-meshheading:12875902-Granulocytes,
pubmed-meshheading:12875902-HL-60 Cells,
pubmed-meshheading:12875902-Humans,
pubmed-meshheading:12875902-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:12875902-Monocytes,
pubmed-meshheading:12875902-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12875902-RNA, Neoplasm,
pubmed-meshheading:12875902-Tretinoin,
pubmed-meshheading:12875902-Tumor Cells, Cultured
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis.
|
| pubmed:affiliation |
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, South Korea.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|