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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-7-22
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pubmed:abstractText |
A major challenge of AIDS research is the development of therapeutic vaccine strategies capable of inducing the humoral and cellular arms of the immune responses against HIV-1. In this work, we evaluated the capability of DCs pulsed with aldrithiol-2-inactivated HIV-1 in inducing a protective antiviral human immune response in SCID mice reconstituted with human PBL (hu-PBL-SCID mice). Immunization of hu-PBL-SCID mice with DCs generated after exposure of monocytes to GM-CSF/IFN-alpha (IFN-DCs) and pulsed with inactivated HIV-1 resulted in a marked induction of human anti-HIV-1 antibodies, which was associated with the detection of anti-HIV neutralizing activity in the serum. This vaccination schedule also promoted the generation of a human CD8+ T cell response against HIV-1, as measured by IFN-gamma Elispot analysis. Notably, when the hu-PBL-SCID mice immunized with antigen-pulsed IFN-DCs were infected with HIV-1, inhibition of virus infection was observed as compared with control animals. These results suggest that IFN-DCs pulsed with inactivated HIV-1 can represent a valuable approach of immune intervention in HIV-1-infected patients.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10075982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10229186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10229227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10553903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10559335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10811870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-10837075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11231634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11336691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11533158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11738745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11745430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11827416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-11839147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-12077267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-12496959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-12734367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-7514684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-8057491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-8892919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-8905100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9302437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9396610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9516110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9551957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9616227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12874266-9863885
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
198
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12874266-AIDS Vaccines,
pubmed-meshheading:12874266-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:12874266-Animals,
pubmed-meshheading:12874266-Dendritic Cells,
pubmed-meshheading:12874266-HIV-1,
pubmed-meshheading:12874266-Humans,
pubmed-meshheading:12874266-Immunomagnetic Separation,
pubmed-meshheading:12874266-Interferon-alpha,
pubmed-meshheading:12874266-Lymphocyte Transfusion,
pubmed-meshheading:12874266-Lymphocytes,
pubmed-meshheading:12874266-Mice,
pubmed-meshheading:12874266-Mice, SCID,
pubmed-meshheading:12874266-Transplantation, Heterologous,
pubmed-meshheading:12874266-Vaccines, Inactivated
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pubmed:year |
2003
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pubmed:articleTitle |
Potent immune response against HIV-1 and protection from virus challenge in hu-PBL-SCID mice immunized with inactivated virus-pulsed dendritic cells generated in the presence of IFN-alpha.
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pubmed:affiliation |
Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena, 299, Rome, Italy 00161.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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