Linked Life Data

12871697

Source:http://linkedlifedata.com/resource/pubmed/id/12871697

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-7-21
pubmed:abstractText
During limb skeletal muscle formation, committed muscle cells proliferate and differentiate in the presence of extracellular signals that stimulate or repress each process. Proteoglycans are extracellular matrix organizers and modulators of growth factor activities, regulating muscle differentiation in vitro. Previously, we characterized proteoglycan expression during early limb muscle formation and showed a spatiotemporal relation between the onset of myogenesis and the expression of decorin, an important muscle extracellular matrix component and potent regulator of TGF-beta activity. To evaluate decorin's role during in vivo differentiation in committed muscle cells, we grafted wild type and decorin-null myoblasts onto chick limb buds. The absence of decorin enhanced the migration and distribution of myoblasts in the limb, correlating with the inhibition of skeletal muscle differentiation. Both phenotypes were reverted by de novo decorin expression. In vitro, we determined that both decorin core protein and its glycosaminoglycan chain were required to reverse the migration phenotype. Results presented here suggest that the enhanced migration observed in decorin-null myoblasts may not be dependent on chemotactic growth factor signaling nor the differentiation status of the cells. Decorin may be involved in the establishment and/or coordination of a critical myoblast density, through inhibition of migration, that permits normal muscle differentiation during embryonic myogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-24
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12871697-Animals, pubmed-meshheading:12871697-Cell Differentiation, pubmed-meshheading:12871697-Cell Line, pubmed-meshheading:12871697-Cell Movement, pubmed-meshheading:12871697-Cell Transplantation, pubmed-meshheading:12871697-Chick Embryo, pubmed-meshheading:12871697-Coculture Techniques, pubmed-meshheading:12871697-Decorin, pubmed-meshheading:12871697-Extracellular Matrix Proteins, pubmed-meshheading:12871697-Gene Expression Regulation, Developmental, pubmed-meshheading:12871697-Genetic Vectors, pubmed-meshheading:12871697-Limb Buds, pubmed-meshheading:12871697-Mice, pubmed-meshheading:12871697-Muscle, Skeletal, pubmed-meshheading:12871697-Myoblasts, Skeletal, pubmed-meshheading:12871697-Myogenin, pubmed-meshheading:12871697-Proteoglycans, pubmed-meshheading:12871697-Retroviridae, pubmed-meshheading:12871697-Transforming Growth Factor beta, pubmed-meshheading:12871697-Transplantation, Heterologous, pubmed-meshheading:12871697-beta-Galactosidase
pubmed:year
2003
pubmed:articleTitle
Inhibition of myoblast migration via decorin expression is critical for normal skeletal muscle differentiation.
pubmed:affiliation
Centro de Regulación Celular y Patología, Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biológicas, MIFAB, P. Universidad Católica de Chile, Santiago, Chile.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't