|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0024202,
umls-concept:C0035253,
umls-concept:C0038952,
umls-concept:C0039194,
umls-concept:C0162597,
umls-concept:C0183683,
umls-concept:C0205100,
umls-concept:C0205263,
umls-concept:C0344211,
umls-concept:C0591833,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1514485,
umls-concept:C1521721,
umls-concept:C1533691,
umls-concept:C1879547
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2003-7-21
|
|
pubmed:abstractText |
Little is known about the homeostatic mechanisms by which the levels of peripheral lymphocytes are maintained. The survival of naïve T cells in vivo must be maintained by some factors that have not been characterized in an in vitro culture system. In this study, we established a culture system of stromal cells derived from murine lymph nodes and investigated the action of the stromal cells in supporting the survival of resting T cells in vitro. Most of the T cells cocultured with the stromal cells did not die, and the supernatant of cultured stromal cells increase the viability of T cells. This T-cell survival-supporting activity was maintained for more than 7 days. Although interleukin (IL)-4, IL-6, IL-7, and interferon-beta also rescued peripheral T cells from spontaneous cell death, medium-soluble and heat-sensitive factor(s) derived from the stromal cells supported the survival of T cells more effectively and for a longer time than did these cytokines. T cells maintained in the culture system with the stromal cells appeared to remain in a resting G0/G1 state and did not show remarkable DNA synthesis. From these results, it is presumed that some soluble factor(s) other than the tested cytokines that have been identified as supporting T-cell survival are produced from lymph node stromal cells. These factor(s) play an important role in maintenance of resting T cells.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10092109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10201896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10233363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10587347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10727454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10784451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-10863978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11034383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11248801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11381057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11527146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11544290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11561182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-11561183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-2328199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-6383006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-7770771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-8344380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-8376790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-8687422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-8848725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9016869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9190930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9197272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9221762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9334361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9334366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9438127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9725205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9808174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12871215-9927514
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0019-2805
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
109
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
496-503
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12871215-Animals,
pubmed-meshheading:12871215-Apoptosis,
pubmed-meshheading:12871215-Cell Division,
pubmed-meshheading:12871215-Cell Survival,
pubmed-meshheading:12871215-Cells, Cultured,
pubmed-meshheading:12871215-Cytokines,
pubmed-meshheading:12871215-DNA,
pubmed-meshheading:12871215-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12871215-Gene Expression,
pubmed-meshheading:12871215-Genes, bcl-2,
pubmed-meshheading:12871215-Immunoblotting,
pubmed-meshheading:12871215-Lymph Nodes,
pubmed-meshheading:12871215-Mice,
pubmed-meshheading:12871215-Mice, Inbred C57BL,
pubmed-meshheading:12871215-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12871215-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12871215-Stromal Cells,
pubmed-meshheading:12871215-T-Lymphocytes,
pubmed-meshheading:12871215-bcl-X Protein
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro.
|
|
pubmed:affiliation |
Departments of Immunology and Equipment Center for Research and Education, Nagoya University Graduate School of Medicine, Nagoya, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
