Linked Life Data

12870845

Source:http://linkedlifedata.com/resource/pubmed/id/12870845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-7-21
pubmed:abstractText
DNA detection is usually performed using fluorescence probes. Using a DNA oligomer stained with the widely used dye 1,1'-[1,3-propanediylbis[(dimethylimino)-3,1-propanediyl]]bis[4-[(3-methyl-2(3H)-benzoxazolylidene)methyl]]-quinolinum tetraiodide (YOYO-1), we show that a substrate containing silver particles can lead to a greater than 10-fold increase in the fluorescence intensity. Proximity to silver particles also increases the photostability of YOYO-1-DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0031-8655
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
604-7
pubmed:dateRevised
2011-5-4
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Increased intensities of YOYO-1-labeled DNA oligomers near silver particles.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA. cfs@cfs.umbi.umd.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.