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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
2003-9-29
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pubmed:abstractText |
Ectodomain cleavage of the ErbB-4 receptor tyrosine kinase generates a membrane-associated fragment of 80 kDa (m80) that has been subjected to N-terminal sequencing. The sequence obtained shows that the N terminus of this fragment begins with Ser-652 of ErbB-4. When a 12-residue peptide corresponding to ErbB-4 residues 646-657 was incubated with recombinant tumor necrosis factor-alpha-converting enzyme, fragments representing residues 646-651 and 652-657 were obtained. These data indicate that ectodomain cleavage of ErbB-4 occurs between His-651 and Ser-652, placing the cleavage site within the ectodomain stalk region approximately 8 residues prior to the transmembrane domain. Several experiments have characterized other aspects of the m80 ErbB-4 fragment. Inhibition of ErbB-4 tyrosine kinase activity with pan-ErbB tyrosine kinase inhibitors indicates that kinase activity is stringently required for heregulin-dependent, but not 12-O-tetradecanoylphorbol-13-acetate-induced, ErbB-4 ectodomain cleavage and formation of the m80 fragment. When the m80 ErbB-4 fragment is generated by cell treatment with heregulin or 12-O-tetradecanoylphorbol-13-acetate, the fragment associates with intact ErbB-2. However, this fragment does not associate with the intact ErbB-4 molecule.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADAM Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/receptor tyrosine-protein kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/tumor necrosis factor-alpha...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38421-7
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:12869563-ADAM Proteins,
pubmed-meshheading:12869563-Amino Acid Sequence,
pubmed-meshheading:12869563-Animals,
pubmed-meshheading:12869563-Binding Sites,
pubmed-meshheading:12869563-Cell Line,
pubmed-meshheading:12869563-Detergents,
pubmed-meshheading:12869563-Humans,
pubmed-meshheading:12869563-Metalloendopeptidases,
pubmed-meshheading:12869563-Mice,
pubmed-meshheading:12869563-Molecular Sequence Data,
pubmed-meshheading:12869563-Peptides,
pubmed-meshheading:12869563-Precipitin Tests,
pubmed-meshheading:12869563-Protein Binding,
pubmed-meshheading:12869563-Protein Structure, Tertiary,
pubmed-meshheading:12869563-Receptor, Epidermal Growth Factor,
pubmed-meshheading:12869563-Recombinant Proteins,
pubmed-meshheading:12869563-Serine,
pubmed-meshheading:12869563-Tetradecanoylphorbol Acetate,
pubmed-meshheading:12869563-Time Factors,
pubmed-meshheading:12869563-Transfection,
pubmed-meshheading:12869563-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment.
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pubmed:affiliation |
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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