Linked Life Data

12868599

Source:http://linkedlifedata.com/resource/pubmed/id/12868599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-7-18
pubmed:abstractText
Epithelial-mesenchymal transition (EMT) may be critical for neoplastic progression and its eventual tumorigenicity of epithelia. In this context, we investigated whether EMT and EMT-associated features occurred after chronic ethanol treatment of human gingival keratinocytes immortalized with the E6/E7 oncogenes of human papillomavirus (HPV) type 16. Following a nine-week treatment of cells with 30 mM ethanol in keratinocyte growth medium, they were cultured in normal DMEM with 10% serum. These cell populations were able to proliferate in this medium gradually exhibiting elongated morphology indicating that these cells underwent EMT. Control cells without ethanol treatment did not survive subcultures in DMEM. Upon long-term subcultures of ethanol-treated cells, two phenotypes were obtained exhibiting epithelium-like and spindle-shape fibroblast-like morphology (respectively, termed as EPI and FIB cells), the latter indicating EMT. In comparison to EPI cells, the phenotypic transition to FIB cells was concomitant with a decrease in the expression of keratins, desmoplakins and a complete loss of K14. Moreover, FIB cell transition strongly correlates with an increase in the expression of vimentin and simple epithelial keratin K18. These alterations in FIB cells were associated with the ability of these cells to exhibit anchorage-independent growth, while EPI cells exhibited anchorage-dependent growth. Concerning the transformation stage, FIB cells represent a progressively more advanced transformed phenotype which may reflect an early step during HPV- and ethanol-dependent multi-step carcinogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-22
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12868599-Animals, pubmed-meshheading:12868599-Cell Division, pubmed-meshheading:12868599-Cell Line, Transformed, pubmed-meshheading:12868599-Cell Transformation, Viral, pubmed-meshheading:12868599-Cell Transplantation, pubmed-meshheading:12868599-Cells, Cultured, pubmed-meshheading:12868599-Epithelial Cells, pubmed-meshheading:12868599-Ethanol, pubmed-meshheading:12868599-Fibroblasts, pubmed-meshheading:12868599-Gingiva, pubmed-meshheading:12868599-Humans, pubmed-meshheading:12868599-Immunoblotting, pubmed-meshheading:12868599-Keratin-14, pubmed-meshheading:12868599-Keratinocytes, pubmed-meshheading:12868599-Keratins, pubmed-meshheading:12868599-Male, pubmed-meshheading:12868599-Mice, pubmed-meshheading:12868599-Mice, Nude, pubmed-meshheading:12868599-Microscopy, Confocal, pubmed-meshheading:12868599-Neoplasms, Experimental, pubmed-meshheading:12868599-Oncogene Proteins, Viral, pubmed-meshheading:12868599-Papillomaviridae, pubmed-meshheading:12868599-Papillomavirus E7 Proteins, pubmed-meshheading:12868599-Repressor Proteins, pubmed-meshheading:12868599-Transplantation, Heterologous, pubmed-meshheading:12868599-Vimentin
pubmed:year
2003
pubmed:articleTitle
Epithelium and fibroblast-like phenotypes derived from HPV16 E6/E7-immortalized human gingival keratinocytes following chronic ethanol treatment.
pubmed:affiliation
Deutsches Krebsforschungszentrum, Department of Applied Tumorvirology, Heidelberg, Germany. Walee.Chamulitrat@med.uni-heidelberg.de
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't