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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2003-7-17
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pubmed:abstractText |
Analyses of early molecular and cellular events associated with long-term plasticity remain hampered in Drosophila by the lack of an acute procedure to activate signal transduction pathways, gene expression patterns, and other early cellular events associated with long-term synaptic change. Here we describe the development and first use of such a technique. Bursts of neural activity induced in Drosophila comatosets and CaP60A Kumts mutants, with conditional defects in N-ethylmaleimide-sensitive fusion factor 1 and sarco-endoplasmic reticulum Ca2+ ATPase, respectively, result in persistent (>4 hr) activation of neuronal extracellular signal-regulated kinase (ERK). ERK activation at the larval neuromuscular junction coincides with rapid reduction of synaptic Fasciclin II; in soma, nuclear translocation of activated ERK occurs together with increased transcription of the immediate-early genes Fos and c/EBP (CCAAT element binding protein). The effect of "seizure-stimulation" on ERK activation requires neural activity and is mediated through activation of MEK (MAPK/erk kinase), the MAPKK (mitogen-activated protein kinase kinase) that functions upstream of ERK. Our results (1) provide direct proof for the conservation of synaptic signaling pathways in arthropods, (2) demonstrate the utility of a new genetic tool for analysis of synaptic plasticity in Drosophila, and (3) potentially enable new proteomic and genomic analyses of activity-regulated molecules in an important model organism.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, Neuronal,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/N-Ethylmaleimide-Sensitive Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sarcoplasmic Reticulum...,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/comt protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/fasciclin II,
http://linkedlifedata.com/resource/pubmed/chemical/kayak protein, Drosophila
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
16
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6362-72
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12867522-Active Transport, Cell Nucleus,
pubmed-meshheading:12867522-Animals,
pubmed-meshheading:12867522-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:12867522-Calcium-Transporting ATPases,
pubmed-meshheading:12867522-Carrier Proteins,
pubmed-meshheading:12867522-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:12867522-Cell Nucleus,
pubmed-meshheading:12867522-Central Nervous System,
pubmed-meshheading:12867522-Drosophila Proteins,
pubmed-meshheading:12867522-Drosophila melanogaster,
pubmed-meshheading:12867522-Gene Expression Regulation,
pubmed-meshheading:12867522-Larva,
pubmed-meshheading:12867522-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:12867522-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12867522-Mutation,
pubmed-meshheading:12867522-N-Ethylmaleimide-Sensitive Proteins,
pubmed-meshheading:12867522-Neuromuscular Junction,
pubmed-meshheading:12867522-Neuronal Plasticity,
pubmed-meshheading:12867522-Neurons,
pubmed-meshheading:12867522-Paralysis,
pubmed-meshheading:12867522-Sarcoplasmic Reticulum Calcium-Transporting ATPases,
pubmed-meshheading:12867522-Seizures,
pubmed-meshheading:12867522-Signal Transduction,
pubmed-meshheading:12867522-Synapses,
pubmed-meshheading:12867522-Temperature,
pubmed-meshheading:12867522-Vesicular Transport Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Acute induction of conserved synaptic signaling pathways in Drosophila melanogaster.
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pubmed:affiliation |
Department of Molecular and Cellular Biology and Arizona Research Labs Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, USA. choeffer@u.arizona.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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