Linked Life Data

12867509

Source:http://linkedlifedata.com/resource/pubmed/id/12867509

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2003-7-17
pubmed:abstractText
By stimulating distinct receptor subtypes, dopamine (DA) exerts presynaptic and postsynaptic actions on both large aspiny (LA) cholinergic and fast-spiking (FS) parvalbumin-positive interneurons of the striatum. Lack of receptor- and isoform-specific pharmacological agents, however, has hampered the progress toward a detailed identification of the specific DA receptors involved in these actions. To overcome this issue, in the present study we used four different mutant mice in which the expression of specific DA receptors was ablated. In D1 receptor null mice, D1R-/-, DA dose-dependently depolarized both LA and FS interneurons. Interestingly, SCH 233390 (10 microm), a D1-like (D1 and D5) receptor antagonist, but not l-sulpiride (3-10 microm), a D2-like (D2, D3, D4) receptor blocker, prevented this effect, implying D5 receptors in this action. Accordingly, immunohistochemical analyses in both wild-type and D1R-/- mice confirmed the expression of D5 receptors in both cholinergic and parvalbumin-positive interneurons of the striatum. In mice lacking D2 receptors, D2R-/-, the DA-dependent inhibition of GABA transmission was lost in both interneuron populations. Both isoforms of D2 receptor, D2L and D2S, were very likely involved in this inhibitory action, as revealed by the electrophysiological analysis of the effect of the DA D2-like receptor agonist quinpirole in two distinct mutants lacking D2L receptors and expressing variable contents of D2S receptors. The identification of the receptor subtypes involved in the actions of DA on different populations of striatal cells is essential to understand the circuitry of the basal ganglia and to develop pharmacological strategies able to interfere selectively with specific neuronal functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
16
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6245-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12867509-Animals, pubmed-meshheading:12867509-Antibody Specificity, pubmed-meshheading:12867509-Blotting, Western, pubmed-meshheading:12867509-Corpus Striatum, pubmed-meshheading:12867509-Dopamine, pubmed-meshheading:12867509-Dopamine Agonists, pubmed-meshheading:12867509-Electrophysiology, pubmed-meshheading:12867509-Interneurons, pubmed-meshheading:12867509-Male, pubmed-meshheading:12867509-Membrane Potentials, pubmed-meshheading:12867509-Mice, pubmed-meshheading:12867509-Mice, Knockout, pubmed-meshheading:12867509-Patch-Clamp Techniques, pubmed-meshheading:12867509-Presynaptic Terminals, pubmed-meshheading:12867509-Protein Isoforms, pubmed-meshheading:12867509-Receptors, Dopamine, pubmed-meshheading:12867509-Receptors, Dopamine D1, pubmed-meshheading:12867509-Receptors, Dopamine D2, pubmed-meshheading:12867509-Receptors, Dopamine D5, pubmed-meshheading:12867509-Receptors, GABA-A, pubmed-meshheading:12867509-Synapses
pubmed:year
2003
pubmed:articleTitle
Receptor subtypes involved in the presynaptic and postsynaptic actions of dopamine on striatal interneurons.
pubmed:affiliation
Clinica Neurologica, Dipartimento di Neuroscienze, Università Tor Vergata, 00133 Rome, Italy.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't