Linked Life Data

12865967

Source:http://linkedlifedata.com/resource/pubmed/id/12865967

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-7-16
pubmed:abstractText
The present study was performed to examine the neuroprotective effects of fangchinoline (FAN) and tetrandrine (TET), bis-benzylisoquinoline alkaloids, which exhibit the characteristics of Ca 2+ channel blockers, on H2O2 -induced neurotoxicity using cultured rat cerebellar granule neurons. H2O2 produced a concentration-dependent reduction of cell viability, which was blocked by (5 R,10 S)-(+)-5-methyl-10,11-dihydro-5 H-dibenzo[ a,d]cyclohepten-5,10-imine (MK-801), an N-methyl- D-aspartate (NMDA) receptor antagonist, verapamil, an L-type Ca 2+ channel blocker, and NG-nitro- L-arginine methyl ester (L-NAME), a nitric oxide synthase (NOS) inhibitor. Pretreatment with FAN and TET over a concentration range of 0.1 to 10 microM significantly decreased the H2O2 -induced neuronal cell death as assessed by a trypan blue exclusion test, a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei. In addition, FAN and TET inhibited the H2O2 -induced elevation of glutamate release into the medium, elevation of the cytosolic free Ca 2+ concentration ([Ca 2+] c ), and generation of reactive oxygen species (ROS). These results suggest that FAN and TET may mitigate the harmful effects of H2O2 -induced neuronal cell death by interfering with the increase of [Ca 2+] c, and then by inhibiting glutamate release and generation of ROS. Abbreviations. AP5:D(-)-2-amino-5-phosphonopentanoic acid DMSO:dimethyl sulfoxide FAN:fangchinoline H 2 DCF-DA:2',7'-dichlorodihydrofluorescin diacetate MK-801:(5 R,10 S)-(+)-5-methyl-10,11-dihydro-5 H-dibenzo[ a,d]cyclohepten-5,20-imine MTT:3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide L-NAME: NG-Nitro- L-arginine methyl ester NMDA: N-methyl- D-aspartate TET:tetrandrine
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0032-0943
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
506-12
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:12865967-Alkaloids, pubmed-meshheading:12865967-Animals, pubmed-meshheading:12865967-Benzylisoquinolines, pubmed-meshheading:12865967-Calcium, pubmed-meshheading:12865967-Cell Death, pubmed-meshheading:12865967-Cerebellum, pubmed-meshheading:12865967-Cytoplasmic Granules, pubmed-meshheading:12865967-Female, pubmed-meshheading:12865967-Hydrogen Peroxide, pubmed-meshheading:12865967-N-Methylaspartate, pubmed-meshheading:12865967-NG-Nitroarginine Methyl Ester, pubmed-meshheading:12865967-Neurons, pubmed-meshheading:12865967-Neuroprotective Agents, pubmed-meshheading:12865967-Phytotherapy, pubmed-meshheading:12865967-Plant Roots, pubmed-meshheading:12865967-Pregnancy, pubmed-meshheading:12865967-Rats, pubmed-meshheading:12865967-Rats, Sprague-Dawley, pubmed-meshheading:12865967-Stephania tetrandra, pubmed-meshheading:12865967-Verapamil
pubmed:year
2003
pubmed:articleTitle
Protective effects of fangchinoline and tetrandrine on hydrogen peroxide-induced oxidative neuronal cell damage in cultured rat cerebellar granule cells.
pubmed:affiliation
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Korea.
pubmed:publicationType
Journal Article