Source:http://linkedlifedata.com/resource/pubmed/id/12865350
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2003-7-16
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| pubmed:abstractText |
The specific biological function of the cell surface or membrane-bound isoform of colony-stimulating factor-1 (mCSF-1) is not well understood. To help define the role of this isoform in bone, we developed a transgenic mouse in which targeted expression of human mCSF-1 in osteoblasts was achieved under the control of the 2.4-kb rat collagen type I alpha promoter. Bone density, determined by peripheral quantitative computed tomography, was reduced 7% in mCSF-1 transgenic compared with that in wild-type mice. Histomorphometric analyses indicated that the number of osteoclasts in bone (NOc/BPm, NOc/TAR, OcS/BS) was significantly increased in transgenic mice (1.7- to 1.8-fold; P < 0.05 to P < 0.01) compared with that in wild-type animals. Interestingly, the osteoblast-restricted isoform transgene corrected the osteopetrosis seen in CSF-1-deficient op/op mice. Skeletal growth and bone density in op/op mice expressing mCSF-1 in osteoblasts were similar to those in wild-type mice and were dramatically different from those in the unmanipulated op/op animals. The op/op mice expressing mCSF-1 in bone had normal incisor and molar tooth eruption, whereas the op/op mice evidenced the expected failure of tooth eruption. These findings directly support the conclusion that mCSF-1 is functionally active in bone in vivo and is probably an important local source of CSF-1.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0013-7227
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
144
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3677-82
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12865350-Animals,
pubmed-meshheading:12865350-Bone Density,
pubmed-meshheading:12865350-Bone Development,
pubmed-meshheading:12865350-Bone and Bones,
pubmed-meshheading:12865350-Cell Count,
pubmed-meshheading:12865350-Collagen Type I,
pubmed-meshheading:12865350-Femur,
pubmed-meshheading:12865350-Gene Expression,
pubmed-meshheading:12865350-Humans,
pubmed-meshheading:12865350-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12865350-Mice,
pubmed-meshheading:12865350-Mice, Inbred C57BL,
pubmed-meshheading:12865350-Mice, Transgenic,
pubmed-meshheading:12865350-Osteoblasts,
pubmed-meshheading:12865350-Osteoclasts,
pubmed-meshheading:12865350-Osteopetrosis,
pubmed-meshheading:12865350-Promoter Regions, Genetic,
pubmed-meshheading:12865350-Rats,
pubmed-meshheading:12865350-Tomography, X-Ray Computed
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| pubmed:year |
2003
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| pubmed:articleTitle |
The cell surface form of colony-stimulating factor-1 is biologically active in bone in vivo.
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| pubmed:affiliation |
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8016, USA. gang-qing.yao@yale.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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