Linked Life Data

12860472

Source:http://linkedlifedata.com/resource/pubmed/id/12860472

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-7-15
pubmed:abstractText
To further elucidate the modulator role of trace metals such as zinc and copper on the activity of nucleotide purinoceptors, the action of these metals was assessed during prolonged ATP applications to rat P2X(4) purinoceptors expressed in Xenopus laevis oocytes. Application of ATP for 3 min resulted in a biphasic effect; a fast transient peak was followed by a slower stable current component with similar pharmacological and biophysical characteristics. The application of 1-300 microM Cu(2+) inhibited both current components to a comparable extent; likewise, Zn(2+) facilitated to a similar degree the transient and the slower stable current components. Carnosine (Car), cysteine (Cys), histidine (His), and the metal chelator, penicillamine, prevented the inhibitory action of Cu(2+); the Zn(2+) facilitation was not prevented by neither Car nor His but by either bathophenantroline or Cys, revealing metal selectivity. While the noncompetitive Cu(2+) inhibition appears to decrease channel conductance, Zn(2+) likely increases ATP affinity independently of the activation state of the purinoceptor. These results strongly support the notion that trace metals modulate the activity of the P2X(4) purinoceptor and could become relevant during continual activity of a P2X(4) purinoceptor-containing synapse.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
472
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-56
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Neuromodulator role of zinc and copper during prolonged ATP applications to P2X4 purinoceptors.
pubmed:affiliation
Unidad de Regulación Neurohumoral, Departamento de Ciencias Fisiológicas, Centro Regulación Celular y Patología, Instituto Milenio MIFAB, P. Universidad Católica de Chile, Casilla 114-D, Stgo. 1, Santiago, Chile.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't