|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2003-7-15
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
114
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
123-34
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:12859903-Binding Sites,
pubmed-meshheading:12859903-Cryoelectron Microscopy,
pubmed-meshheading:12859903-Eukaryotic Cells,
pubmed-meshheading:12859903-Models, Molecular,
pubmed-meshheading:12859903-Molecular Conformation,
pubmed-meshheading:12859903-Peptide Elongation Factor G,
pubmed-meshheading:12859903-Peptides,
pubmed-meshheading:12859903-Protein Biosynthesis,
pubmed-meshheading:12859903-Protein Structure, Tertiary,
pubmed-meshheading:12859903-Protein Subunits,
pubmed-meshheading:12859903-RNA, Messenger,
pubmed-meshheading:12859903-RNA, Transfer,
pubmed-meshheading:12859903-Ribosomes,
pubmed-meshheading:12859903-Rotation
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Locking and unlocking of ribosomal motions.
|
|
pubmed:affiliation |
Howard Hughes Medical Institute, and Health Research Incororated at the Wadswoth Center, State University of New York, Albany, 12201, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
