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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
38
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pubmed:dateCreated |
2003-9-15
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pubmed:abstractText |
Recent studies have suggested that the NF-Y transcription factor is involved in transcription repression of the cell cycle regulatory genes in a response to p53 induction or DNA damage. Here we demonstrate the cdk2-dependent phosphorylation of NF-Y and its involvement in transcription repression by the p53-p21 signaling pathway. Cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y. Cyclin A-cdk2 appears to associate with NF-Y both in vitro and in vivo. Furthermore, YA protein is phosphorylated in parallel with a cell cycle-dependent activation of cdk2 kinase and cyclin A expression. YA phosphorylation is unnecessary for heterotrimer formation with the YB-YC dimer. However, NF-Y containing a phosphorylation-deficient mutant form of YA, YA-aa, has its DNA binding activity impaired. Consistently, YA-aa inhibits transcription activation of a NF-Y target promoter, cdc2, by cdk2. These results facilitate the elucidation of the regulatory mechanisms of cell cycle progression involving the p21-cdk2-NF-Y signaling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Binding Factor,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin A,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/roscovitine
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
36966-72
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12857729-Amino Acid Sequence,
pubmed-meshheading:12857729-CCAAT-Binding Factor,
pubmed-meshheading:12857729-CDC2-CDC28 Kinases,
pubmed-meshheading:12857729-Cell Cycle,
pubmed-meshheading:12857729-Cell Line,
pubmed-meshheading:12857729-Cell Nucleus,
pubmed-meshheading:12857729-Cyclin A,
pubmed-meshheading:12857729-Cyclin-Dependent Kinase 2,
pubmed-meshheading:12857729-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:12857729-Cyclins,
pubmed-meshheading:12857729-DNA Damage,
pubmed-meshheading:12857729-Dimerization,
pubmed-meshheading:12857729-Glutathione Transferase,
pubmed-meshheading:12857729-Humans,
pubmed-meshheading:12857729-Molecular Sequence Data,
pubmed-meshheading:12857729-Mutation,
pubmed-meshheading:12857729-Phosphorylation,
pubmed-meshheading:12857729-Plasmids,
pubmed-meshheading:12857729-Precipitin Tests,
pubmed-meshheading:12857729-Promoter Regions, Genetic,
pubmed-meshheading:12857729-Protein Binding,
pubmed-meshheading:12857729-Purines,
pubmed-meshheading:12857729-Serine,
pubmed-meshheading:12857729-Signal Transduction,
pubmed-meshheading:12857729-Time Factors,
pubmed-meshheading:12857729-Transfection,
pubmed-meshheading:12857729-Tumor Suppressor Protein p53
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pubmed:year |
2003
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pubmed:articleTitle |
Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway.
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pubmed:affiliation |
National Research Laboratory, Department of Microbiology, Dankook University College of Medicine, Cheonan, 330-714, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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