Linked Life Data

12855724

Source:http://linkedlifedata.com/resource/pubmed/id/12855724

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2003-7-11
pubmed:abstractText
Pathogenic bacteria in the family Neisseriaceae express surface receptors to acquire iron from the mammalian iron-binding proteins. Transferrins and lactoferrins constitute a family of iron-binding proteins highly related in both sequence and structure, yet the bacterial receptors are able to distinguish between these proteins and uphold a strict binding specificity. In order to understand the molecular basis for this specificity, the interaction between human lactoferrin (hLf) and the lactoferrin-binding protein A (LbpA) from Moraxella catarrhalis was studied. A periplasmic expression system was designed for the heterologous expression of LbpA, which enabled the investigation of its binding activity in the absence of lactoferrin-binding protein B (LbpB). To facilitate delineation of the LbpA-binding regions of hLf, chimeric proteins composed of hLf and bovine transferrin were made. Binding studies performed with the chimeric proteins and recombinant LbpA identified two binding regions within the C-terminus of hLf. Furthermore, native LbpA from Moraxella and Neisseria spp. bound the identical spectrum of hybrid proteins as the recombinant receptor, demonstrating a conserved binding interaction with the C-lobe of hLf.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1729-37
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12855724-Amino Acid Sequence, pubmed-meshheading:12855724-Animals, pubmed-meshheading:12855724-Bacterial Outer Membrane Proteins, pubmed-meshheading:12855724-Bacterial Proteins, pubmed-meshheading:12855724-Binding Sites, pubmed-meshheading:12855724-Carrier Proteins, pubmed-meshheading:12855724-Cattle, pubmed-meshheading:12855724-Humans, pubmed-meshheading:12855724-Lactoferrin, pubmed-meshheading:12855724-Molecular Sequence Data, pubmed-meshheading:12855724-Moraxella (Branhamella) catarrhalis, pubmed-meshheading:12855724-Protein Structure, Tertiary, pubmed-meshheading:12855724-Receptors, Cell Surface, pubmed-meshheading:12855724-Recombinant Fusion Proteins, pubmed-meshheading:12855724-Recombinant Proteins, pubmed-meshheading:12855724-Sequence Homology, Amino Acid, pubmed-meshheading:12855724-Transferrin
pubmed:year
2003
pubmed:articleTitle
Bacterial lactoferrin-binding protein A binds to both domains of the human lactoferrin C-lobe.
pubmed:affiliation
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Rm 274, Heritage Medical Research Building, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't