Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2003-7-10
pubmed:abstractText
To study the relationship between DNA replication and transcription in vivo, we investigated Hox gene activation in two vertebrate systems: the embryogenesis of Xenopus and the retinoic acid-induced differentiation of pluripotent mouse P19 cells. We show that the first cell cycles following the mid- blastula transition in Xenopus are necessary and sufficient for HoxB activation, whereas later cell cycles are necessary for the correct expression pattern. In P19 cells, HoxB expression requires proliferation, and the entire locus is activated within one cell cycle. Using synchronous cultures, we found that activation of HoxB genes is colinear within a single cell cycle, occurs during S phase and requires S phase. The HoxB locus replicates early, whereas replication is still required for maximal expression later in S phase. Thus, induction of HoxB genes occurs in a DNA replication-dependent manner and requires only one cell cycle. We propose that S-phase remodelling licenses the locus for transcriptional regulation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10068998, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10101128, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10319820, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10479456, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10805758, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10885747, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-10891496, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-11511348, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-11588253, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-12067662, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-12355067, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-12399303, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-12432383, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-12432398, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1689682, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1794324, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1811161, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1901716, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1909569, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-1975088, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-2806112, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-6183003, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-6719109, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7139712, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7481802, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7813770, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7900990, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7913880, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-7985240, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-8756292, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-8922526, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-8973154, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-9281333, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-9284351, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-9631657, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-9671588, http://linkedlifedata.com/resource/pubmed/commentcorrection/12853488-9794816
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3737-48
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Vertebrate HoxB gene expression requires DNA replication.
pubmed:affiliation
Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Cedex 05 Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't